Effects Of Phosphate And Organic Carbon On Soil Microbial Community And Diversity In Typical Antarctic Regions

In this study,samples were taken from the Kitezh Lake,Half-point,the Great Wall Station Square,Airport North and Geography Bay in the Fildes Peninsula,Antarctica,and the soil samples collected from the Antarctic region were subjected to bacterial and fungal isolation,purification,count of colony,and phylogenetic identification of16S rRNA genes and ITS.At the same time,16S rRNA v3-v4 high-throughput sequencing of the soil samples from the Kitezh Lake region and the semi-three-point regionto was conducted to analyze with the microbial diversity and composition.Then,combined with the results of the Antarctic soils environmental factors,the relationship between Antarctic soil microbial diversity and organic carbon,and the relationship between microbial diversity and phosphate was analyzed.The experimental results showed that a total of 33 strains of culturable bacteria and6 strains of culturable fungi were isolated from 15 soil samples of 5 Antarctica typical regions sampling points.The identified bacteria 33 strains belonged to Actinobacteria,Proteobacteria and Acidobacteria.The six fungi belong to the genus Zygomycota(3strains)and the Ascomycota(3 strains).High-throughput sequencing results showed that the soil in the Kitezh Lake area and the semi-three-point area was rich in bacteria,and the Shannon index of the bacteria in the Kitezh Lake region was 7.65-9.5.The most abundant bacteria are Actinobacteria and Proteobacteria in the Kitezh Lake region.The index of bacterial ACE in the semi-three-point region is2279.775-3779.903 and the goods_coverage index ranges from 0.978 to 0.988.The most abundant ones are Proteobacteria and Acidobacteria.Based on the results of the Antarctic soil environmental factors,the correlation analysis of microbial communities showed that there was a strong correlation between soil bacterial community composition and the soil environmental factors in the meltwater of Kitezh Lake.Among them,organic carbon(r~2=0.68,P<0.05)is the most relevant to soil bacterial communities in Antarctica Kitezh Lake and has a greater impact on soil microbes.Hungatei decomposes organic carbon and exhibits chemotactic behavior.Phosphate addition was selected in the semi-three-point area soils.The results showed that spraying phosphate affected the bacterial community and diversity in the soil.Soil pH and water content are also significantly associated with soil bacterial communities.Sphingolipid bacteria can synthesize sphingolipids using phosphorus,and bacterial enrichment is susceptible to phosphate concentrations.