Effects Of Fe(?) On The Diversity Of Bacteria And AutotropHic Carbon Fixation Bacteria In Paddy Soils

The seasonal flooding and drainage process of paddy soil affect the existence of iron elements,Fe(?)/Fe(?)is the main redox system of paddy soil,and its morphological transformation affects the redox nature of paddy soils,which also affects the distribution of bacterial community diversity.Bacterial community,as a functional group,is closely related to people's production and life.The changes of bacterial community structure will inevitably lead to functional changes.The abundance of iron in the crust rank fourth,which also largely included in the paddy soils.Iron cycle always coupling with others chemical reaction,such as nitrogen cycle,carbon cycle.Clear the effect of Fe(?)on the diversity distribution of bacteria and autotrophic Carbon fixation bacteria is helpful to understand the role of Fe(?)in the change of bacterial community,and then guide the agricultural production and improve the soil environment.Analyzed by fluorescence quantitative technique and Illumina MiSeq sequencing technique and the results show that the effect of Fe(?)addition on 16S rRNA gene copies number level and bacterial conmmunity abundance level is not significance,High Fe(?)addition can significantly reduce the diversity of L2 bacterial community;The low addition of Fe(?)could significantly reduce the diversity of S1 and S2 bacterial communities;Fe(?)addition treatment can significantly reduce the cbbM copies number in S1 and S2 samples,but not significantly affect the abundance of different treated samples of loam soils and sandy soils.Based on the OTU level PCA analysis and based on the sequence level unweight unifrac non-similarity distance matrix analysis showed that LCK and L1 are close to L2 and SCK is close to S1 and S2 in the bacterial community OTU and sequence similarity level.S1 and S2 are close to LCK and L1 in OTU composition and sequence similarity of autotrophic Carbon fixation bacteria.16S rRNA gene high-throughput sequencing phylum level analysis,a total of 32 phyla,define the percentage abundance of more than one thousandth as dominant phyla a total of 19 dominant phyla catched.The significance analysis indifferent treated sample showe that the change of Actinobacteria percentage in loam is LCK<L1<L2,Bacteroidetes percentage change is L2<L1<LCK,The percentage change in Firmicutes and Cyanobacteria is LCK<L1 = L2,Acidobacteria percentage change is LI = L2<LCK,Euryarchaeota percentage change is LCK = L1<L2,Chlorobi percentage changes is L2<L1 = LCK,The percentage change in the percentage of Ignavibacteriae is LCK<L1>L2,Verrucomicrobia percentage change is LCK>L1<L2.The percentage change of Firmicutes in the sandy is SCK<S1<S2.Proteobacteria,Actinobacteria,Bacteroidetes,Gemmatimonadetes,Planctomycetes percentage change is SI = S2<S1,The percentage change of Cyanobacteria is SCK>S1<S2.LEfSe analysis was used to dominant genus.In the loam,the genus biomarker in different treated samples play a important role in Actinobacteria,Gemmatimonadetes,Bacteroidete,Ignavibacteriae,Actinobacteria percentage change,Proteobacteria,Firmicutes,Cyanobacteria,Euryarchaeota,Planctomycetes percentage change and so in Proteobacteria,Actinobacteria,Bacteroidetes,Gemmatimonadetes,Planctomycetes,Cyanobacteria percentage change in sandy soil.Fe(?)has a significant effect on transport function in loam and sand,and high Fe(?)addition significantly reduces other functions,the Fe(?)addition in the sand can significantly increase the Calvin cycle,and the addition of Fe(?)in the loam can significantly increase the passage of the Calvin cycle.In the metabolic pathway,the addition of Fe(?)in loam can significantly increase the nitrogen fixation pathway,and the addition of high Fe(?)in sandy soil can significantly increase the denitrification and ammoxidation pathway.In the methane metabolism,the addition of high Fe(?)in the loam can significantly increase the methane production The addition of Fe(?)in thirography can significantly reduce the collateralized sulfur reduction pathway,increase the alienated sulfur reduction pathway,and the high Fe(?)addition in sandy soil can significantly reduce the assimilated sulfur reduction pathway.Got three phyla in cbbM high-throughput sequencing,namely Proteobacteria,Cyanobacteria,Actinobacteria,and Proteobacteria is the dominant phylum which average percentage abundance is more than 96%.Got 8 class,namely Alphaproteobacteria,Betaproteobacteria,Gammaproteobacteria,Deltaproteobacteria,and Alphaproteobacteria,Betaproteobacteria,Gammaproteobacteria is the dominant class.Got 16 genus,The abundance of Thiobacillus in LCK Significantly higher than L1 and L2;The abundance of Rhodopseudomonas in L1 Significantly higher than LCK and L2;The abundance of Acidihalobacter in L2 Significantly higher than LCK and L1;The abundance of Magnetospirillum in SCK Significantly higher than S1 and S2.