Molecular Phylogenetics Of Partial Chiroptera Groups In Guizhou Based On Mitochondrial 12S RRNA Gene

Guizhou lies in southwest of China and about 7 families 17gemera and 40 species of bats inhabit here. Except Rousettus leschenaultia, all of them are microbats (Wang, 2003; Luo, 1995). 12S rRNA gene for 17 bat species in 3 families (Rhinolophidae, Hipposideridae, Vespertilionidae) from Guizhou were sequenced, along with the corresponding sequences of 13 species in the same three families from Genbank, to deduce inter and intra relationships of these bats. In order to deduce whether Rhinolophidae and Hipposideridae should belong to microchiroptera or Yinpterochiroptera and to explain the status of subfamily Miniopterinae deeply, the sequences of 3 species in family Pteropodidae and 1 species in family Molossidae were also obtained from Genbank. Chosin three species of Bovidae as outgroup, MP analyse was performed for all the five Chiroptera families. The results indicated that Rhinolophidae and Hipposideridae were closely related to Pteropodidae, indicating the paraphyly of microchiroptera. The relationships among Vespertilionidae, Rhinolophidae and Hipposideridae were further deduced by Bayesian ME and NJ methods. The results clearly demonstrated: 1. Subfamily Miniopterinae should be elevated to family status. 2. Myotis should be promoted to subfamily Myotinae, and was sister to the clade containing Kerivoulinae and Murininae. 3. Myotis should be subdivided according to New and Old world species instead of ecomorph. 4. Myotis altarium was not closely related to M. myotis and M. davidii phylogeneticly. 5. Ia probably belonged to group Eptesicini instead of Pipistrellini in Pipistrellus-like bats. 6. Aselliscus stoliczkanus belongs to Hipposideridae other than Rhinolophidae. 7. In the gunus Hipposideros, Hipposideros armiger claded wiht H. larvatus firstly, then with H. pratti; 8) Rhinolophus.sp grouped with R. ferrumequinum and separated from other Rhinolophids firstly followed by R. affinis. monoceros and R. blythi as well as R. rex grouped together respertively.