The Separation Of Fusarium From Wheat In Huanghuai Area And Control Its Toxins

The main food of most people in China is derived from wheat.Wheat and its products are found everywhere in daily life.The safety of these foods is closely related to our health and safety.In recent years,due to the increase of rainfall and the change of climate,Huanghuai wheat district has frequent occurrence of head blight and wheat security has been greatly threatened.Wheat These cereal crops are susceptible to Fusarium during growth and storage.Fusarium is currently considered to be the leading cause of wheat head blight,and a series of Fusarium toxins produced by Fusarium will be directly dangerous to us.health.Among them,deoxynivalenol(DON,DON)is the most common mycotoxins,and it is also the most widely contaminated toxin in wheat.There are some Fusarium toxins although there is no serious DON pollution,but also worth our attention,such as zearalenone(ZEN),snow rot fungus alcohol(NIV),acetylated DON(3A-DON,15A-DON)As well as DON-?-D-glucoside(D3G),these toxins are often overlooked in the daily monitoring and control process,but they also cause certain harm to the human body after entering the human body and become a potential safety hazard in food.In this study,130 wheat samples collected from 3 provinces and 12 cities in the Huanghuai wheat region were subjected to risk assessment.Through the detection of six mycotoxins,dietary exposure assessments were conducted for different sex populations,and these toxins were determined to affect the human body in daily life.The hazards;Secondly,the separation of Fusarium from wheat samples,and identification of Fusarium toxicity,determination of the relationship between Fusarium and mycotoxins in wheat,through the further optimization of toxigenic conditions after the control of toxins in wheat hazards;the final use of probiotics The bacterium that removes the highest DON poisoning rate from the bacteria is optimized for the removal conditions and it is expected to control the DON toxin and apply it to daily production.The results of the study are as follows:(1)Analysis of six common Fusarium toxins(DON,3A-DON,15A-DON,NIV,ZEN,and D3G)in 130 wheat samples from the Huanghuai region indicate that DON,D3 G,ZEN,and 15A-DON are four toxins There was pollution,and the detection rates were 63.1%,29.2%,3.8%,and 2.3%,respectively.Among them,only DON exceeded the standards,which were based on China's and European Union's limit standards,and the rate exceeded 6.9% and 9.2%.(2)The dietary exposure assessment results showed that the average intakes of the four toxins DON,D3 G,ZNE,and 15A-DON in the low-age group were higher than those in the high-age group,and the average intake of toxins in the female children in the low-age group.Larger than male children of the same age group,whereas the older age group had the opposite result.The risk assessment results show that DON poisoning in wheat in Huanghuai area is a certain risk,and the current risk of the other three toxins is at a controllable and safe level.(3)8 strains of Fusarium spp.were isolated and identified from 130 wheat samples from Huanghuai wheat area.Among them,3 strains were non-toxigenic strains,and the remaining 5 strains could produce Fusarium toxin(DON,3A-DON,15-DON).),but all are non-high-producer strains.The production of 3A-DON and 15A-DON was more affected by environmental impact than DON,and the production of 15A-DON changed the most,and there was a significant difference in the amount of toxin produced by the same strain under different conditions.(4)Lactobacillus rhamnosus(L7,L8)and Lactobacillus plantarum(21801,21805)have a prominent inhibition on the growth of Fusarium,and they can inhibit the spore germination of Fusarium to varying degrees,and the longer the culture time,the more The rate is higher;it has a significant inhibitory effect on the growth of Fusarium mycelium,and can maintain a significant inhibition of mycelium growth before 96 hours,the inhibition range is between 31-39%,co-culture to 144 h,and the control group Compared with no obvious inhibitory effect.The optimal conditions for the removal of DON from lactobacillus were as follows: culture time 6 h,culture temperature 36 o C,initial p H=6,culture rotation speed 200 rpm,clearance rate over 75%.