Effects Of Rhizospheric Microorganisms On Populations Of Scutellaria Tsinyunensis

Scutellaria tsinyunensis C.Y.Wu et Chow is an endemic plant in Chongqing.It is only isolated on an ?island? in the Jinyun Mountains.In recent years,the populations and the number of plants have been decreasing,and they are now in an endangered state.The endangered causes of endangered plants include internal factors such as restricted heritability and fertility,and external factors such as environmental,human and other interference.The rhizosphere microorganisms that are closely related to the root system of plants have an extremely important role in plant growth and development.Based on the principle of interaction between soil microorganisms and plant roots,this study used high-throughput sequencing technology to specifically determine the microbial diversity in the rhizospheric soil of the different populations of S.tsinyunensis on the Jinyun Mountain.The analysis is to provide soil microbiological basis for the growth of S.tsinyunensis.The research results are as follows:1.The V4-V5 region of 16 S rDNA was selected for high-throughput sequencing.A total of 456,365 effective bacterial sequences and 289,010 optimized sequences were obtained from the rhizosphere soils of 9 S.tsinyunensis populations.A total of 12-30 phylum,28-39 class,50-69 order,and 63-99 family,97-174 genus,and 109-200 species bacteria were identified,of which the LuoyangqiaoII(B76)population identified the least bacterial composition;Baiyun Zhuhai(B41)has the most abundant bacterial composition.Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Planctomycetes,Chloroflexi,Gemmatimonadetes and Nitrospirae were the main groups of rhizospheric soil populations in each population of S.tsinyunensis.They have a certain influence on the growth of S.tsinyunensis;among them,the abundance of Proteobacteria in each population was more than 30%.2.The bacterial community structure of each population was affected by environmental factors and soil physical and chemical properties,and the degree of impact was: Population Area>Elevation>Aspect > Canopy density>Slope,PH>Organic matter> Available phosphorus >Water content > Alkaline nitrogen.The most abundant Proteobacteria was positively correlated with canopy density(0.01< P <0.05);Acidobacteria was negatively correlated with elevation(0.01< P <0.05);Bacteroidetes Positive correlation with elevation was observed(0.01< P <0.05);Actinobacteria was positively correlated with population area(0.01< P <0.05);Planctomycetes was positively correlated with elevation(0.001< P <0.01).3.The order of bacterial community diversity in the rhizosphere soils of 9 populations was as follows: Helongyuan(B42)> Baiyun Zhuhai(B41)> Shizi feng(B77)> Luoyangqiao(B40)> Shihuasi(B74)> Shaolongguan(B43)> Beiwenquan(B61)> Qinglongzhai(B75)> LuoyangqiaoII(B76).Beta diversity analysis showed that the bacterial diversity in the rhizosphere soil of 9 populations of S.tsinyunensis was divided into two groups: Shihuasi(B74),Shizifeng(B77),Helongyuan(B42),Baiyunzhuhai(B41)as a category.Beiwenquan(B61),LuoyangqiaoII(B76),Qinglongzhai(B75),Shaolongguan(B43),and LuoyangqiaoII(B76)are a class that shows the difference in bacterial diversity between rhizosphere soils..4.High-throughput sequencing of the soil fungal genome ITS sequence was performed.The amount of sequencing data was reasonable and basically reflected the biological information of the actual sample.A total of 496179 fungal valid sequences and 418106 optimized sequences were obtained from rhizosphere soil samples of 9 populations of S.tsinyunensis,and 6-9 phylum,15-23 classes,31-56 orders and 48-107 family,67-172 genus,75-195 species were identified;of which Shaolongguan(B43)identified the least fungi,Luoyangqiao(B40)had the most abundant populations;Ascomycota,Basidiomycotina,Zygomycota,Glomeromycota,Chytridiomycota,Rozellomycota and Blastocladiomycota are the main flora of nine populations of fungal communities.Bacterial/fungal species of Helongyuan(B42)were more abundant than Luoyangqiao II(B76)in the two returning populations,and they were similar to the diversity of wild populations in the rhizosphere bacterial/fungal community,showing more suitable for the regressive growth of Astragalus membranaceus.5.The order of the fungal community diversity in the rhizospheric soil of the 9 populations was as follows: Helongyuan(B42)>Shizifeng(B77)>Baiyun Zhuhai(B41)> Shihuasi(B74)>Qinglongzhai(B75)>Luoyangqiao(B40)>Beiwenquan(B61)>Shaolongg uan(B43)>Luoyangqiao(B76).Beta diversity analysis showed that 9 populations were not significantly classified,but it could be concluded that the fungal diversity in the rhizosphere soils of the two populations of the Shizifeng(B77)and Shihuasi(B74)species was the most similar.6.The fungal community structure of each population was affected by environmental factors and soil physicochemical properties,and the degree of impact was as follows: elevation> canopy density> slope direction> population area> slope,PH> alkaline nitrogen> available phosphorus> organic matter > Water content.The most abundant Ascomycota was positively correlated with the PH value(0.01< P <0.05).