The Process Of Five Biochemical Substances For Regulating Growth And Metabolism Of Chlorella Vulgaris

Chlorella sorokiniana is a photoautotrophic organism that synthesizes valuable metabolites and can accumulate specific,high-value metabolites?such as lipids,proteins,polysaccharides,and pigments?under different culture conditions.These products can be used for the production of cosmetics,foods,health products,medicines,etc.,thereby giving C.sorokiniana more and more attention.However,the content of these metabolites in the cells of C.sorokiniana is low and it lacks a cost-effective method of separation and purification,which greatly reduces the degree of industrialization.This study intends to use biochemical regulation to increase the accumulation of metabolites of C.sorokiniana and explore relevant mechanisms.The nutrients that can be used in the environment can be divided into four major categories:carbon source,nitrogen source,inorganic salt,and auxin.After preliminary experiments,inositol,sodium acetate?carbon source?,and sodium selenite?inorganic salts?,glycine?carbon source+nitrogen source?,indomethacin?plant hormones?for research.Using batch mode with measuring the C.sorokiniana biomass,pigment content,chlorophyll fluorescence,the pH value of the culture solution,and the content of metabolites?polysaccharides,proteins?after the culture,the growth and metabolite accumulation influences of C.sorokiniana.Below are key research findings:1.Among the selected substances,glycine had the most significant promoting effect on the growth of C.sorokiniana.When the concentration was 8 g/L,the number of C.sorokiniana was 6.50 times that of the control group.Followed by sodium acetate,inositol,indomethacin and sodium selenite.2.Effect on the pH of the culture:pH of the culture will gradually increase after adding inositol,sodium acetate,and sodium selenite to the culture;pH of the culture with glycine and indomethacin will decrease.However,the pH of the culture gradually increases with the incubation time.3.Chlorophyll content of C.sorokiniana:After adding inositol,glycine,indomethacin and sodium selenite can increase the chlorophyll content of C.sorokiniana,the highest were 1.80,2.20,1.40,2.30 times the control group;After addition of sodium acetate,the chlorophyll content of Chlorella decreased significantly,with a minimum of 70%of the control group.4.Effects on the photosynthetic efficiency of C.sorokiniana:After adding inositol,indomethacin and sodium selenite,the?_PSIISII of C.sorokiniana decreased significantly.The lowest values were 87%,24%,and 66%of the control group,respectively.5.The most obvious accelerating effects on the accumulation of oil,protein and polysaccharide content of C.sorokiniana were sodium acetate,glycine and inositol,which were 12.19,4.50,and 1.40 times that of the control group,respectively.