The Effects Of Simulated Flue Gases On The Growth And Lipids Accumulation Of Chlorella Sorokiniana CS-01

Since the green-house effect was mainly caused by carbon dioxide in the atmosphere which largely coming from fossil fuel burning, carbon dioxide emission reduction has become the key of controlling the green-house effect. Meanwhile, due to the depleting supplies of world for petroleum sourced fuels, more and more people recognize that it is necessary to develop renewable, eco-friendly transport fuels for environmental and economic sustainability. Biodiesel derived from biomass of algae is a potential renewable fuel source and has great capability of application in terms of their higher biomass production, faster growth and higher lipid content, etc. At present, due to the expensive source, biodiesel is not widely used in the world. In this respect, carbon dioxide emission reduction and new energy was connected by microalgal biotechnology to reduce production costs. Therefore, this study mainly focuses on the effects of simulated flue gases on the growth and lipids accumulation of Chlorella sorokiniana CS-01.The results showed that Chlorella sorokiniana CS-01could grew at simulated flue gas containing15%CO2and grew well under CO2concentrations ranging from5%-10%. Chlorella sorokiniana CS-01showed a higher biomass content (1.1and1.2gL-1, respectively) and the dry cell biomass are3.3times and2.5times higher than the control. At the same time, the results indicated that aerating with simulated flue gases could greatly improve the lipids content, ranging from28%to43%of dry weight of biomass, and the cell lipids content is1.5times higher than the control under simulated flue gas containing15%CO2. The main fatty acid compositions of strain CS-01were C14-C18(>72%) short-chain FAMEs (known as biodiesel feedstocks), meanwhile, the biodiesel productivity was over60%, suggesting that Chlorella sorokiniana CS-01has a great potential for CO2mitigation and biodiesel production. Furthermore, differential expression of three genes related to CO2fixation and fatty acid synthesis were studied to further describe the effect of simulated flue gases on the growth and lipid cummulation of strain CS-01at molecular level, indicating that the expression of gene related with concentrations of CO2in simulated flue gases, heteromeric ACCase rather than homomeric ACCase is responsible for the synthesis of lipid in strain CS-01.5%,10%and15%CO2affect the distribution of carbon flow.The research set up a relative accurate cell amount determining method—CD680nm absorption photometry, by contrasting turbidimetry, spectrophotometry, and thrombocytometry. Through comparing several methods for lipids extraction, a better modified method was established for further lipids extraction. Statistics analysis was measured using SPSS17.0and the all the results well supported the experimental outcomes and conclusions.