MiR-301b~miR-130b-PPAR? Axis Underlies The Adipogenic Capacity Of Mesenchymal Stem Cells With Different Tissue Origins

Objective: Comparison of adipogenic differentiation of mesenchymal stem cells(MSCs)from three different sources of human bone marrow,fat,and umbilical cord,to reveal the molecular mechanism of adipogenic differentiation ability from the molecular aspects of microRNAs and gene expression.Providing a theoretical basis for clinical treatment and prevention of obesity and a series of related metabolic disorders.Methods: Isolate and cultivate mesenchymal stem cells derived from human adipose,bone marrow,and umbilical cord.The surface antigens of three different sources of cells were detected by flow cytometry,and these cells are then induced to differentiate into adipocytes.The three tissues-derived MSCs adipogenic differentiation was analyzed through oil red staining after cultured for 14 days.We tested the expression of miR-27 and miR-130/miR-301/miR-454 cluster by quantitative real-time PCR(qRT-PCR)at 14 d of differentiation,and detected the mRNA expression levels of PPAR?,FABP4,PLIN1 and LPL simultaneously.Through analysis of the correlation between PPAR? and miR-27 and miR-130/mi R-301/mi R-454 family members in three different sources of mesenchymal stem cells in adipogenic differentiation of mesenchymal stem cells,and to explore the potential mechanism ofdifferences in adipogenic differentiation ability.Results: There results showed that the UC-MSCs,AD-MSCs andBM-MSCs displayed similar morphology and immunophenotypes.The adipogenic potential of these MSCs was different,AD-MSCs exhibited the highest adipogenic potential,UC-MSCs displayed the lowest,while potential of BM-MSCs get between;The qRT-PCR detection showed that the expression level of PPAR? was the lowest in UC-MSCs and the highest in Ad-MSCs,while expression level in BM-MSCs get between,these results were identical with the RT-PCR.At the same time,the results of miR-27,miR-130 a,miR-130 b,miR-301 a,miR-301 b,and mi R-454 of three sources of mesenchymal stem cells during adipogenic differentiation was BM-MSC > Ad-MSC > UC–MSC,BM –MSC > UC-MSC > Ad-MSC,UC-MSC > Ad-MSC > BM–MSC,UC-MSC > BM-MSC > Ad-MSC,UC-MSC > BM-MSC >Ad-MSC and BM-MSC > UC-MSC > Ad-MSC.Based on the above analysis of the correlation between mi RNAs and PPAR? expression,we found that miR-301 b and mi R-130 b are located on the same chromosome and form miR-301b~mi R-130 b cluster,which expresses PPAR? in three sources of mesenchymal stem cells.And the lipid-forming capacity of the three cells showed a significant negative correlation.Conclusion: Three different sources of mesenchymal stem cells have significant differences in their ability to induce differentiation intoadipocytes,and their adipogenic differentiation capacity is negatively correlated with the expression of miR-301b-mi R-130b-PPAR? axis.The miR-301b-miR-130b-PPAR? axis involved in determining the adipogenic potential of mesenchymal stem cells from different tissues.