Effects Of Salt Stress And Poplar ERF37 On Expression Of Transcription Factor Genes From Arabidopsis Thaliana

Transcription factor,also known as trans-acting factor,can interact specifically with cis-acting elements in the eukaryotic gene promoter region,activate or inhibit transcription by interacting with each other or with other related proteins,then promote expression of downstream target genes.Expression of its downstream genes is in act as the role of the switch.Different transcription factors usually have different function area.The difference between plant transcription factor according to its structural domain can be divided into WRKY,bZIP,EIL,MYB,NAC,GATA,AP2/ERF,SBP transcription factors family,etc.Transcription factors play an important role in plant growth and development,plant organ formation,control of plant secondary metabolism and hormone response signal transduction.In this study,analysis of genetically modified Arabidopsis salt-tolerant ability was based on transgenic of poplar ERF37 gene in Arabidopsis thaliana in the prophase,and the effects of salt stress and exogenous ERF37 gene on the expression level of Arabidopsis thaliana transcription factor gene were analyzed by RNA-sequencing.Results are as follows:(1)The germination experiment,growth traits,analysis of physiological index,show that seed germination rate,root length,fresh weight,POS activity and SOD activity in genetically modified Arabidopsis with poplar ERF37 gene are significantly better than that of the wild-type under salt stress,while MDA content significantly lower than wild-type Arabidopsis.Transgenic poplar ERF37 gene in Arabidopsis did better in salt tolerance.(2)Analysis of RNA-sequencing in Arabidopsis thaliana under salt stress and non-stress shows that 493 transcription factor genes are founded as differentially expressed.Among them,281 genes are up-regulated and 212 genes are down-regulated.These differentially expressed genes are mainly distributed in 41 families of transcription factors.17 transcription factor families are founded no differentially expressed genes.There are four transcription factors family containing more than 40 differentially expressed genes,and in turn is ERF,bHLH,MYB,NAC.(3)Analysis of RNA-sequencing in genetically modified Arabidopsis and non transgenic Arabidopsis shows that 206 transcription factor genes are founded as differentially expressed.Among them,88 genes are up-regulated and 118 genes are down-regulated.These differentially expressed genes are mainly distributed in 34 families of transcription factors.24 transcription factor families are founded no differentially expressed genes.There are four transcription factors family containing more than 40 differentially expressed genes,and in turn is ERF,bHLH,MYB,NAC.These differentially expressed genes are mainly distributed in transcription factors family.There are four transcription factors family more than 20 differentially expressed genes,and in turn is MYB,NAC,ERF,bHLH.The result shows that the fold change of the gene expression level of endogenous transcription factor in the wild type was normal distribution along 1 under the salt stress.After the poplar ERF37 gene was transferred,the fold change of the gene expression level of endogenous transcription factor is normal distribution along 0 when it is no more than 0.The results showed that there was no correlation between gene expression under salt stress physical and chemical properties of protein.The strong response transcription factors are high thermal stability transcription factor proteins.