Molecular Genetic Interaction Between AGL16 And MYB44 And Its Effect On Flowering And Adaptation In Arabidopsis Thaliana

Climate change seriously threatening the stability and biodiversity of the global ecosystem.As the cornerstone of the ecosystem,plants must adjust their own growth and development to deal with climate and environmental changes,so as to reach the maximum degree of fitness;Choosing the appropriate flowering time,when to exchange from vegetative growth to reproductive growth,is critical for achieving its maximum fitness and for species long-term survival.The Brassicaceae-specific mi R824-AGL16 module regulates flowering time via interacting with SVP and other flowering inhibitory factors and then affect downstream gene expression of FT.While its specific mechanism is still unclear.Previous work in our laboratory showed that AGL16 can potentially bind to the chromatin of MYB44,which is involved with stress adaptation.Based on this,the present study focuses on the molecular genetic interaction of AGL16-MYB44 and its effects on flowering adaptation.The main results are as follows:1.Chromatin immunoprecipitation combined with high-throughput sequencing and quantitative PCR revealed significant binding of AGL16 to MYB44 chromatin;2.Gene expression analysis marked that AGL16 inhibits transcription of MYB44;3.Genetic analysis demonstrated with Col-0,agl16-1,myb44-1,m3(m3 is a mi R824 overexpression material),agl16-1;myb44-1,m3;myb44-1 early flowering and agl16-1;myb44-1,m3;myb44-1 and myb44-1 flowering phenotype is consistent,therefore MYB44 is located downstream of mi R824-AGL16 module during flowering regulation;4.AGL16-MYB44 interaction affected dormancy and seed germination,and the flowering response to high temperature induction;5.Stomata movement behaviors were significantly affected by both AGL16 and MYB44.In summary,this report confirms the direct interaction between AGL16 and MYB44,reveals the its roles in fine-tuning the biological processes during floral transition and seed germination.The additionally constructed genetic materials should provide more resources for further investigation.Our work puts the AGL16-MYB44 in the gene-regulatory-network of flowering time control,and identifies a potential molecular link between floral transition and environmental stress.This work also provided a possibility for clarifying the important role of mi R824-AGL16 module in plant evolution.