Effects Of Biological 3D Printed PLGA Scaffolds On Cell Behavior And Function

Three-dimensional(3D)cell culture is an in vitro cell culture technique simulating 3D growth of tissue cells in vivo.The 3D cell growth in the body not only provides the physical support,but also plays an irreplaceable role in the transmission of biochemical signals and the exchange of genetic information.In recent years,many researchers have devoted themselves to the study of 3D porous nanomaterials with good mechanical properties and good biocompatibility.The development of the biological 3D printing technology could provide technical support for the research and verification of the cell behavior mechanism.This research studied the construction of poly(lactic acid)copolymer(PLGA)fiber scaffold by biological 3D printing,and compares it with microsphere culture and the growth metabolism curve and apoptosis rate of two-dimensional cell culture cells.The 3D PLGA scaffolds were used for cell culture to build a three-dimensional environment for cell growth.The migration mechanism of cancer cells was verified and studied by using the 3D culture system.Through the cell transfection,cell migration,protein immunoblotting and other experiments,the results showed that the expression of p53 protein affects the migration of colon cancer cells after cultured on PLGA scaffolds.Further molecular biological results show that the expression of p53 protein is positively correlated with the expression of MMP-2 protein.This experiment provides a theoretical basis for further study of the migration of cancer cells.The 3D cultrure system was also used to guide the proliferation and differentiation of skeletal muscle cells.Through immunofluorescence,immunoblotting and ce ll proliferation and adhesion experiments,the effect of PLGA monolayer fiber scaffold on the differentiation of C2C12 cells,and the effect of PLGA multilayer fiber scaffold on the penetration of C2C12 cells were studied.Results show that the fibrous scaffold could accelerate the differentiation speed of skeletal muscle cells and promote the repair of skeletal muscle damage.In summary,a 3D cell culture model was constructed by using biological 3D printing,which can be used for the in vitro study of cell behaviors and functions,and tissue engineering.