| The development of renewable and carbon neutral biofuels has attracted tremendous attentions because of the exhaustion of fossil fuels and global warming.Microalgae have been regarded as one of the most promising feedstocks that can potentially address the twin challenges of energy security and environmental protection.Microalgae also has certain advantages compared to other energy crops,including a high growth rate,short growth time,high biomass production,and low land use.Monoraphidium sp.FXY-10 has been regarded as a promising oleaginous algae,and studies including screening of media cultures,assessments of accumulation of microalgae biomass and lipid,and enzymatic hydrolysis of cell wall are pursued in this paper to provide a reference of scale-up lipid usage.The results are showed below:1.Increase of biomass accumulation was observed in culture CZ-M1 and Kuhl,while decreased in MCM and SE.Highest biomass was found in CZ-M1.2.Effects of fulvic acid on growth of microalgae were noticed when added into the medium.Compared with BG11,increment of microalgae biomass increased from 12.34%up to 22.44%when FA was added at 30 mg/L.3.Cellulose,combining with pectinase and alkaline protease showed significant effects on hydrolysis of microalgae cell wall.The lipid yield was achieved at 58.8%under optimum conditions,which were temperature of 30?,time of 10 h and pH value of 6.4.Pretreatment of thermal acid before enzymatic hydrolysis improved the efficiency of lipid extraction. |
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