The Ultrastructure And Dinstinct Primary Sensory Presentation To Airflow And Airborne Odor In The Antennal Sensory Organs Of BACTROCERA CORRECTA

Insects profoundly affect human well-being.Its Pollination behavior directly affects the problem of biodiversity and food security.Its oviposition behavior endangers the crops and forests.The insect vector-borne diseases threat human health.So,how to detection,locate food sources,mate has always been focused on.A large number of studies have shown that airflow is used as the basic signal by insects and odor is used as the second key signal for sources location.However,the neural mechanisms responsible for detecting airflow and odor stimuli remains unclear.Using SEM technique,sensilla type,type character,sensilla distribution were observed in the male guava fruit fly antenna.Antennal sensilla were grouped into six major types,including that Trichoid,Basiconic,Clavate,Grooved,Arista/Johnston's organ,Microtrichia?Two new type sensilla were found and were charateritics of the combination of pit and nodulus.Based on the evidence of the transition from the nodulus to pit,we first give the hypothesis about the formation mechanism of pore allows odor to pass.Based on electroantennography-related literature since 1957,it is well believed that one time depolarization responses occurs when several species antenna are exposed to the airflow using electroantennography(EAG)technique.However,for the first time,we found a kind of new signal characteristics when the fruit fly are exposed to the airflow.B The airflow on evoked three times depolarization response,including that 20 ms airflow evoked the first time depolarization response,40 ms airflow evoked the second time depolarization response,more than 100 ms airflow evoked the third time depolarization.Interestingly,subsequent the airflow off evoked the fourth depolarization.This is the first time report that the antenna has the ability to process airflow-related neural information for four times.The new combinatorial neural mechanism for airflow detection may be responsible for the adaption to the unstability of airflow.In order to further clarify the EAG reponse of the fruit fly when exposed to odor.We observed methyl eugenol evoked EAG response in the male guava fruit fly.Methyl eugenol could evoke excitation and inhibition of the antennal neurons.In conclusion,our experimental results illustrates the combinatorial neural coding mechanism for airflow and odor occur.This break through the people's understanding of the original,and help the development of pesticides of interfering airflow detection,and contributes to the research of chemical ecology.