Study On Mouse Embryo Cardiac Hypertrophy Caused By Influenza Virus H1N1 Infection And Its Differently Expressed Genes Screening

Congenital heart disease is one of the highest birth defects in human mortality,of which 8-10%of the family hereditary congenital heart disease,the rest for the distribution of congenital heart disease.The interaction of Genetic and environmental is the main cause of congenital heart disease,the environmental factors that including drugs,environmental pollution,intrauterine infection and so on.Influenza virus as one of the environmental factors of intrauterine infection,has been of concern.It has been reported that H1N1 fragment protein interacts with a variety of functional proteins in host cells,which in turn affects the expression of the host and viral proteins at the translation level.To investigate the effect of HINI influenza virus on neonatal mouse heart abnormalities,SPF Kunming white mice is used by this research,and is infected influenza virus in 7.5 days pregnant mice.The results showed that the phenomenon of cardiac hypertrophy occurred in the paraffin sections of the heart of newborn mice,and further confirmed that HIN1 could cause cardiac hypertrophy during embryonic development by anatomical photograph and calculation of heart weight-to-weight ratio.In order to explore its molecular mechanism in depth,25 up-regulated genes and 2 genes were down-regulated by high-throughput sequencing and bioinformatics analysis in 11.5 days of embryonic development.The expression of camk4 and grap2 was up-regulated.This study provides a preliminary theoretical basis for H1N1-induced cardiac hypertrophy,which lays a foundation for further study of its molecular mechanism.