Analysis Of Physiological Response And Resistance Gene CcSnRK2 Cloning And Sequencing Of The Gene Source Of Jute Drought Stress

Drought stress is one of the most important environmental problems in the current global,all around the world land area,about 34%of the area was located in the arid and semi-arid state,the proportion of China is close to 50%,the lack of water directly affects the increase of crop production.To improve the drought resistance of crop is more urgent and important,and the research of drought resistance mechanism in plants is the premise and foundation.Jute,one of the most important fiber crops,is an important raw material of textile industry.For the bast fiber plants had strong adaptability and high fiber yield,fiber quality characteristics to adapt a variety of application requirements,so it had a huge potential for development.In this study,screening of strong drought resistance jute varieties as the premise,to explore different genotype jute the osmotic regulation and active oxygen metabolism of change in indoor simulated drought stress condition,We had selected 11 varieties of strong drought resistant jute and 30 parts drought weaker jute germplasm,in order to provide the basis for future study.The selection of drought resistant cultivar IJO20,179(1)and the weak drought tolerance variety 179(7)and Hezi 4 were used as the experimental materials for the further osmotic adjustment and active oxygen metabolism.2.Under the condition of PEG6000 simulated drought stress,relative water content,proline,soluble sugar,MDA content and superoxide dismutase,peroxidase and catalase activity in leaves of 4 jute genotypes including IJO20,179(1),179(7)and Hezi 4 were measured to explore osmotic regulation and reactive oxygen metabolism changes in jute.With drought stress time prolonging,soluble sugar content,free proline content,peroxidase activity in leaves of jute genotypes vigorous growth period increased,and the varieties with strong drought tolerance changed significantly;The content of MDA increased with the stress and the increase range of weak drought resistance varieties 179(7)and Hezi 4 were more significant;The relative water content of the leaves was decreased and the reduce amplitude of weak drought resistance varieties were more significant;The change of superoxide dismutase activity is the first to decrease and then increase;But the activity of catalase was first increased and then decreased.3.SnRK2 family members play a role in response to plant stress.In this study strong drought resistant varieties of jute IJO20 were selected as the experimental materials and homologous cloning and RACE technology were used to clone homologous SnRK2 from jute,1579bp CDS,encoding 350 amino acids.Speculated that encode a protein with a molecular formula of C3736H6248N1218O1572S217,relative molecular weight about for 31.6KD,predicted PI values for 548,has a half-life of about 100h,the instability index was 39.09,belonging to the stability of protein.The protein contains positively charged residues(Arg + Lys)27,37 of negatively charged residues(ASP and Glu),the total average hydrophobicity index for-0.215,fat soluble index is 89.10 prediction showed that the protein is soluble protein.Using fluorescence quantitative PCR analysis of jute under drought stress.With the change of time,the gene CcSnRK2 expression increased and then decreased.In the face of drought stress showed that the CcSnRK2 gene plays a role in jute.