The Immune Toxicities Of Hormone Like Environmental Pollutants In Early Life Stages Of Model Fish

Environmental hormone-like pollutants are one kind of environmental endocrine disrupting chemicals(EDCs)that often detected in the aquatic systems.Recently,the effects of environmental hormone-like pollutants on fish sex determination and its immune system is becoming the hot topic of environmental science.They can not only interfere with the endocrine system of fish,but also disrupt the development and function of immune system.Among EDCs,the steroid hormone 17?-ethynylestradiol(EE2)is widely used as contraceptive pills,while 17?-trenbolone(TB)is a synthetic anabolic hormone extensively used for growth promotion in animal feedlot.In this study,RNA-Seq and PCR were used to investigate the gene expression pattern and gender differences at different developmental time point of Japanese medaka(Oryzias latipes).Then we explored the effects of EE2 and TB on the immune system.The results showed that at embryonic and larval period of medaka,the expression of genes involved in nerve and development were significantly changed,while the gender specific expression for the development of immune system were obviously displayed.The transcription level of complement related genes in females was higher than that in males.The environmental hormone-like pollutants could interfere with the transcription of immune gene in juvenile medaka.EE2 caused a significant inhibition of complement-related genes,and high concentration of EE2 could induce the transcription of cytokines.TB exposure caused an inhibitory effect on the complement system in males,and interfered with the complement system in females as well.Moreover,TB exposure at high concentration induced transcription of cytokines.These results showed that hormone-like pollutants could disrupt the immune system in medaka,which is helpful for the accurate evaluation of the ecological toxicity of hormone-like pollutants.