| The current model for eukaryotic tRNA gene transcription is that two required factors, TFIIIB and TFIIIC, bind to two small (;This model however is not sufficient to explain recent experimental results. In particular, in vitro analysis of a Bombyx mori tRNA;In this dissertation we demonstrate that, contrary to the current model, tRNA gene transcription depends on four components; the polymerase and factors TFIIIB, TFIIIC, and an additional factor, TFIIID. We show that all three factors TFIIIB, TFIIIC, and TFIIID, but not the polymerase, contribute to formation of stable transcription complexes. Additionally, we demonstrate that, in contrast to expectations from current models, no single component can bind to the gene. Binding requires interaction among two or more factors. This suggests a new model, namely that a multicomponent complex of factors interacts with a large region of DNA to form the stable transcription complex. We further demonstrate that only combinations of factors containing TFIIID can bind to the tRNA... |
