Functional Analysis Of Protein Kinase Gene GmRLK1 And Transcription Factor Gene GmTFB3 In Soybean

Plants are inevitable to suffer from the different environment stresses in their growth and development,and have evolved the capacity to respond to wide range of stresses.Protein kinases and transcription factors play vital roles in plant response to abiotic and biotic stresses.For Protein kinases,they can affect the signaling transduction by phosphorylating the substrate proteins.And transcription factors can enhance or repress the expression of genes related to stress tolerance by combining the cis-acting element in the promoter.In a word,Protein kinases and transcription factors are indispensable in the mechanism of plant response to stresses.Abiotic stresses,such as drought,high salt,and low temperature,have a strong impact on crop growth and development and production.It can increase the ability of plant resistance to stresses and the amount of crop by exploring,identifying,and using genes related to stress.Early work obtain a series of candidate genes through yeast two hybrid screening libraries for protein kinase GmY82 associated with drought resistance and salt tolerance.This study chose two genes related to stresses as the research object,one is GmRLK1 encoding receptor protein kinase and another is GmTFB3 encoding transcription factor which contain B3 domain structure.This experiment studied the function of GmRLK1 and GmTFB3 in plant response to adversity stress and discusses the possibility of these two genes involving in the GmY82 related signaling pathways.The major results are as following:1.The function analysis of GmRLK1 in soybean.Drought and salt induced the expression of soybean GmRLK1.It found that under the condition of NaCl and PEG treatment,GmRLK1 is down-regulated by analyzing the characteristic of GmRLK1 gene expression.It found that under the condition of NaCl and PEG treatment,the germination rate of transgenic GmRLK1 Arabidopsis thaliana is lower than the germination rate of mutant.But,soybean GmRLK1 can improve the frost resistance of transgenic Arabidopsis.It found that under the condition of low temperature,GmRLK1 is down-regulated.And the survivals of the transgenic Arabidopsiswere superior to the survival of mutant plants.2.The function analysis of GmTFB3 in soybean.Under the ABA and NaCl treatment,germination rate of transgenic Arabidopsis is lower than the germination rate of mutants.This shows that the soybean GmTFB3 may participate in the salt and ABA stress response.3.The interactions relations between GmRLK1,GmTFB3 and GmY82.It found that GmRLK1 and GmTFB3 can interact with GmY82 by verifying through the protein interaction techniques: yeast double cross,double molecule fluorescence complementary(BiFC)and GST pull-down.This suggests that GmRLK1 and GmTFB3 may have a certain function in GmY82 related signaling transduction.This revealed that GmTFB3 may be involved in plant response to salt and ABA stress path.This experiment found that GmY82 interactions proteins play important roles in abiotic stresses,and this is conducive to further study the mechanism of its action and provides a theoretical support to apply in wheat and other crops.