The Transformation Of Drosophila Melanogaster Defensin's Gene Function And The Resistance Research Of CG13422,Dro,Dpt,MtK

Antimicrobial peptides are insects infected with bacteria,the blood cells and fat cell secretion in instantaneous antibacterial material.Broad-spectrum antimicrobial activity and could kill drug-resistant strains,etc.Antibacterial peptides not only wide antimicrobial spectrum,and for some fungi and protozoa,virus and tumor have kill and inhibition,also can accelerate the immune and wound healing.Defense is a kind of widespread in nature,small molecules with microbial resistance of antimicrobial peptides.Through the directional genetic modification technology,make specific defense gene gain greater long-acting antibacterial and bacteriostatic effect of advantage.To CG13422,droz,Dpt,and at the same time the MtK these genes to study the antimicrobial properties,using the PCR technology,flies to total DNA as a template,amplify the ends joined the joint defense genes,joint processing into sticky end,the defense genes and secretory type yeast expression vector pHBM905A restructuring,to construct recombinant yeast expression vector pHBM905A/defensin,by "sandwich" sandwich plate filter and colony PCR identification certificate purpose gene has been integrated into the yeast chromosome.Choosing positive clones after alcohol induced 1 of expression product by sds-page electrophoresis analysis,finally by agarose diffusion method to detect the biological activities of clear liquid.Defense,fixed-point modification got good antimicrobial resistance strain,but extend the very weak performance.CG13422,droz,Dpt and MtK this several possible resistance genes had not found that the antimicrobial properties,but for later reference basis is provided for the experiment.