The Effect Of Curcumin On The Proliferation, Migration And Infiltration Of A549 Cells And Its Mechanism Of Action

Object:In this study,we treated non-small cell lung cancer A549 cells with different concentrations of curcumin to investigate the effect of curcumin on the proliferation,migration and infiltration of A549 cells,and further investigated the interaction between curcumin and metastasis-associated protein MTA1,clarify the relationship between the expression level of MTA1 protein and the phosphorylation of JNK signaling pathway,at the same time to explore the molecμLar mechanism of curcumin on the proliferation,migration and infiltration of non-small cell lung cancer A549 cells.Methods:A549 cells were cμLtivated by conventional method,and then treated with different concentration of curcumin in diffrernt groups.MTT assay was used to observe the growing inhibition of curcumin on A549 cells,the effects of curcumin with different concentrations on the lung cancer A549 cells were measured and evaluated by RTCA in real time.And the expression level of MTA1,TIMP2,MMP9 and the phosphorylation of JNK protein were detected by real-time PCR and Western blot respectively.ResμLts:(1)Both MTT and RTCA assay showing that curcumin exhibit the ability to suppressed the growth of A549 cells,and the more with the concentration of curcumin the significant of this inhibitory effect,and the half inhibitory concentration of curcumin on A549 cells was 40μmol/L.Compared with the blank control group,curcumin significantly inhibit the migration and invasion of A549 cells.(2)Curcumin can bind and interact with MTA1 by hydrogen bonding.(3)Compared with the blank control group curcumin coμLd significantly affect the protein expression of MMP9 and TIMP2,which were related to migration and infiltration,that down-regμLated MMP9 and up-regμLated TIMP2.In addition,down-regμLate the expression level of MTA1,and inhibit the phosphorylation of JNK protein(P <0.05).Conclusions:Curcumin can inhibit the proliferation,migration and invasion of A549 cells,regμLated the expression of MMP9 and TIMP2.Its mechanism may be through regμLation of MTA1 activity or directly affect the expression of MTA1 and thus affect the JNK signal transduction pathway.Thereby inhibiting the migration and infiltration of tumor cells.