The Relationship Between The Expression Of ESRP1 And E-cadherin In Gastric Cancer And Clinicopathological Features

Objective: This article aimed at exploring the function and possible mechanism of ESRP1 and E-cadherin in the whole progress of gastric cancer by studying the expressions of ESRP1 and E-cadherin in human gastric cancer and analyzing the relationship between its expressions and clinicopathological features of gastric carcinoma and prognosis.Methods: 60 cases of surgically resected gastric carcinoma tissues and its complete pathology and 20 cases of normal gastric mucosa tissues were collected.Through detecting the expression of ESRP1 and E-cadherin in gastric cancer tissues by immunohistochemical method,the relationship between their expressions,clinicopathological features and prognosis can be analyzed in using SPSS19.0 software.By the transient transfection of gastric cancer SGC-7901 cells and exogenous expression of ESRP1,the changes of gastric cancer cell migration can be observed.Through detecting the changes of expression between ESRP1 eukaryotic expression vector transfection and EMT related protein E-cadherin,N-cadherin in blank vector transfection group SGC-7901 cells by Real-time PCR,the effects of ESRP1 expression on EMT in gastric cancer cells can be further illustrated.Results: Immunohistochemical results were analyzed statistically.In differentiation and invasion degree of gastric cancer and the occurrence of lymph node metastasis in these pathological features,the difference of ESRP1 expression was statistically significant(P<0.05);and the pathological features and the expression of ESRP1 are collected(P<0.05).In gastric cancer cells with high differentiation and no breakthrough in the lamina propria and without lymph metastasis,the difference between increasing expression of E-cadherin and low differentiation,breakthrough lamina propria,and lymph node metastasis was statistically significant(P<0.05);the degree of differentiation,invasion degree and lymph node metastasis of gastric cancer are related to the expression of E-cadherin(P<0.05).The positive expression rate of ESRP1 in 60 cases of gastric carcinoma tissue was about 55%.The positive expression rate of ESRP1 in normal gastric mucosa was 40%.So the difference was not statistically significant(P>0.05).The positive expression rate of E-cadherin in 60 cases of gastric carcinoma tissue was about 60% and the positive expression rate of E-cadherin in normal gastric mucosa tissue of the patients was 100%(P<0.05)in 20 cases.The positive expression rate of ESRP1 and E-cadherin in gastric carcinoma tissue was about 43.3%.Spearman correlation analysis showed that in gastric cancer tissue,The expression of E-cadherin was positively correlated with the expression of ESRP1(P<0.001).COX multivariate regression analysis showed that the expression of ESRP1 and lymph node metastasis were independent prognostic factors in patients with gastric cancer after radical gastrectomy(P0.05).Cell scratch test results showed that the ESRP1 plasmid was transfected into SGC-7901 group than the control group SGC-7901 plasmid was transfected into gastric cancer cell migration ability decreased significantly(P<0.05);Real-time PCR results showed that the ESRP1 eukaryotic expression carrier transfuses the more blank carrier transfection group E-cadherin in expression quantity rises,N-cadherin expression quantity decreases(P<0.05).Conclusion: ESRP1 and E-cadherin are obviously related with invasion,differentiation of gastric cancer and lymph node metastasis;ESRP1 and E-cadherin expression in gastric cancer tissue are related;the expression of ESRP1 in the gastric cancer is an independent prognostic factor;the overexpression of ESRP1 in the gastric cancer SGC-7901 cell can affect the ability of gastric cancer cell to be removed by promoting E-cadherin expression and inhibiting N-cadherin expression to affect EMT.