| Acne vulgaris is the result of multifactorial processes in and around the pilosebaceous unit.However,there are a signifi cant number of adults and adolescent who also suffer from acne.Genetic factorsmay determine the failure of acne-pronefollicles to normally evolve into acne-resistant follicles in early adult life,as well asenviromient.Increased sebum excretion,alteration of lipidcomposition and the oxidant/antioxidant ratiocharacteristic of the skin surface lipids are majorconcurrent events associated with the develop-ment of acne,Free fatty acids were found tobe elevated in skin surface lipid from patients with inflammatory acne.as well as,P.acnes is a commensal anaerobic,gram-positive rod whose interaction with the innate immune systems plays a signifi cant role in acne development.sebum interferes with theprocess of follicular keratinisation in the pilose-baceous unit,pore blockage may occur,contrib-uting to lesion formation and acne.Acne is a disease of infl ammation mediated by the innate immune system at the level of the pilosebaceous unit.as well as,wind-heat?liver depression and damp_heat syndrome were a common disease in traditional Chinese medicine.Therefore,that prescription of the yu ning ointment is determined.the prescription that includ Anemone flaccid.Spica Schizonepetae?Chinese parsnip root?cortex dictamni and Sophora flavescens,there have effect in dispelling pathogenic wind,relieving exterior syndrome,soothing liver,and removing dampness.To study the pharmacodynamics of acne immune pathogenesis.1.To establite the replication of acne modelObjective:To establite of a C57 mouse model of acne on the backMethods:The mouse of the unmated C57 tha have twenty,It have half male and half female.We randomly divided it into control groups and 9 gradient concentration oleic acid modules,We divided it into two groups.We will shave off the hair on the back of the mice about 2 cm*2 cm before the experiment.control groups:We smeared the mouse back with100%liquid paraffin about 60ul every day,I will keep smearing 21 days.oleic acid groups:We smeared the mouse back with 9 gradient concentration oleic acid(25.5%?100.0%,k=0.85)about 60ul every day.I will keep smearing 21 days.we will kill the mice on the 22 days and will take back skin.as well as paraffin section.Pathological changes of skin were observed by hematoxylin-eosin(HE)staining.The diameter of sebaceous glands and ratio of follicular keratinization area and was morphometric analyzed.The median effective concentration(EC50)were analyzed with non-linear various slope regression using Prism-5.01 software.Results:The lesion of the mouse gradually aggravated With an increase in oleic acid dose.EC50 of oleic acid groups were 65%.Y=0.09+0.84/[1+10(26.93-14.87x)],R=0.98,IC50 was 42.07?99.74%.The results suggest that We have successfully established the mouse of a C57 acne model.Conclusion:Oleic acid can cause sebaceous gland hyperplasia and can cause epithelial keratosis in the funnel of hair follicle,as well as can sebum discharge is not smooth,Eventually forming acne.Previous studies have used animal ears as modeling sites,But the ear lacks a complete follicular unit,It can't completely show acne lesions.So In this experiment,the back with abundant hair follicles was used as the model site.It is similar to the patient.2.Dose-effect of Yuling ointment and its disassembled prescription in treating acneObjective:By conducting animal experiments to clarify the dose-effect relationship of yuning ointment and its disassembled prescription in the treatment of acne.At half the effective dose that the dose of drug causing 50%positive reaction in the subject to determine Anemone flaccida as a key drug for the curative effect of the prescription,so we take the prescription compared with the Anemone flaccid to observe the compatibility and synergism.Methods:By animal experiments,the mouse of the unmated C57 tha have 120,It have half male and half female.We randomly divided it into control groups and 9 gradient concentration oleic acid modules,We divided it into two groups.We will shave off the hair on the back of the mice about 2 cm*2 cm before the experiment.control groups:We smeared the mouse back with100%liquid paraffin about 60ul every day,I will keep smearing 21 days.oleic acid groups:We smeared the mouse back with 65%oleic acid about 60ul every day.I will keep smearing 21 days.At 22 days,when build a successful model,We randomly divided it into 22 groups,he gradient dosage of Anemone flaccida crude drug dose(1.06?1060.23mg·kg-1·d-1,k=3.16),Yu-ning ointment decomposed Anemone flaccida crude drug dose(4.73?1767.75 mg·kg-1·d-1,k=3.16),and Yu-ning ointment crude drug dose(2.84?2827.28 mg·kg-1·d-1,k=3.16),and the control group and model group Applying matrix,that were administrated for 14 days,respectively.Pathological changes of skin were observed by hematoxylin-eosin(HE)staining.The diameter of sebaceous glands and ratio of follicular keratinization area and was morphometric analyzed.The serum IL-1,IL-6,and TNF-? levels were detected by Elisa assay.At 35 days,after 4h,we use the chloral hydrate anesthesia,and We took a blood abdominal aorta andperfusion fixed,will take back skin.as well as paraffin section.Pathological changes of skin were observed by hematoxylin-eosin(HE)staining.The diameter of sebaceous glands and ratio of follicular keratinization area and was morphometric analyzed.The serum IL-1,IL-6,and TNF-? levels were detected by Elisa assay.The median effective dose(ED50)were analyzed.with non-linear various slope regression using Prism-5.01 software.Results:With the Anemone flaccid to return,To improve the sebaceous glands hypertrophy and hair follicle keratinization cathetersand collagenous fiber,the ED50 of Yuning ointment was Anemone flaccid 0.28,0.14,7.20 times.lowerIL-1,IL-6and TNF-? secretion.the ED50 of Yuning ointment was Anemone flaccid 0.15,0.49 and 0.24 times.The intestinal propulsion rate and white blood cell count were 9.65,18.0 times.With the Yuning ointment crude Anemone flaccid to return.To improve the sebaceous glands hypertrophy and hair follicle keratinization cathetersand collagenous fiber and werIL-1,IL-6 and TNF-?secretion,the ED50 of Yuning ointment less than the ED50 of Yuning ointment crude Anemone flaccid.Conclusion:In mice induced by oleic acid acne model,group Dose-effect of Yuling ointment and its disassembled prescription in treating acne,we find thatYuling ointment and its disassembled prescription can improve the sebaceous glands hypertrophy and hair follicle keratinization cathetersand collagenous fiber and werIL-1,IL-6and TNF-? secretion,Yuling ointment was best.3.Yuling ointment efficacy for the treatment of acneObjective:To oberve Yuling ointment efficacy for the treatment of acneMethods:We will shave off the hair on the back of the mice about 2 cm*2 cm before the experiment.control groups:We smeared the mouse back with 100%liquid paraffin about 60ul every day,I will keep smearing 21 days.oleic acid groups:We smeared the mouse back with 65%oleic acid about 60ul every day.I will keep smearing 21 days.At 22 days,when build a successful model,We randomly divided it into 7 groups,the Model group?Isotretinoin Gel group(ATRA,60 mg·kg-1·d-1).tacrolimus group(TAC,60 mg·kg-1·d-1)?the Anemone flaccida group(DW,60mg·kg-1·d-1),the low Yu-ning ointment group(YNL,60mg·kg-1·d-1),group,and the middle Yu-ning ointment group(YNM,60mg·kg-1·d-1)?and the high Yu-ning ointment group(YNM,60mg·kg-1·d-1),we will take back skin.At 36 days,we will take back skin.as well as paraffin section.Pathological changes of skin were observed by hematoxylin-eosin(HE)staining.The diameter of sebaceous glands and ratio of follicular keratinization area and was morphometric analyzed.The serum IL-1,IL-6,and TNF-? levels were detected by Elisa assay.Results:Compared with the control group,Model group mice can improve hair follicle keratinization(P<0.01),can improve exdermoptosis(P<0.01),can improve sebaceous glands around collagen deposition(P<0.05),and can improve IL-1(P<0.01)?IL-6(P<0.01)andTNF-?(P<0.01)secretion.Compared with the model group,Positive medicine of Isotretinoin Gel and tacrolimus can lower hair follicle keratinization(P<0.01),can lower exdermoptosis(P<0.05),can lower sebaceous glands around collagen deposition(P<0.05),and can lower IL-1(P<0.05)?IL-6(P<0.05)and TNF-?(P<0.05)secretion.Compared with the Positive medicine of Isotretinoin Gel and tacrolimus can can lower hair follicle keratinization(P<0.01),lower exdermoptosis(P<0.01),can lower sebaceous glands around collagen deposition(P<0.05),and can lower IL-1(P<0.01)?IL-6(P<0.05)and TNF-?(P<0.05)secretionConclusion:Yuling ointment can can lower hair follicle keratinization,can lower exdermoptosis,can lower sebaceous glands around collagen deposition,and can lower IL-1and TNF-? secretion4.Yuling ointment immune pharmacological mechanism of prevention and treatment of acne Objective:To oberve Yuling ointment immune pharmacological mechanism of prevention and treatment of acneMethods:We will shave off the hair on the back of the mice about 2 cm*2 cm before the experiment,control groups:We smeared the mouse back with100%liquid paraffin about 60ul every day,I will keep smearing 21 days,oleic acid groups:We smeared the mouse back with 65%oleic acid about 60ul every day.I will keep smearing 21 days.At 22 days,when build a successful model,We randomly divided it into 7 groups,the Model group?Isotretinoin Gel group(ATRA,60 mg·kg-1·d-1).tacrolimus group(TAC,60 mg·kg-1·d-1)?the Anemone flaccida group(DW,60mg·kg-1·d-1),the low Yu-ning ointment group(YNL,60mg·kg-1·d-1),group,and the middle Yu-ning ointment group(YNM,60mg·kg-1·d-1)?and the high Yu-ning ointment group(YNM,60mg·kg-1·d-1),we will take back skin.At 36 days,Expression of IL-1??TNF-? and JNK were determined with immunohistochemical staining,and Western blot.Results:Yuling ointment a can lower the IL-1??TNF-? and JNK secretion,expression of protein?gene level and protein level.Conclusion:Yuling ointment efficacy for the treatment of acne was best... |
PDF Full Text Request