Effect And Mechanism Of Ginseng Polysaccharide On NK92-MI Cell Killing Activity

Purpose:Ginseng is the dried root or rhizome of Araliaceae.It has the functions of nourishing vitality,returning to blood pressure,nourishing Qi in spleen and lungs,nourishing blood and fluid in the body,soothing the nerves and heiping with wisdom.Ginseng polysaccharide is one of the main components of ginseng,which has the functions of enhancing immunity,anti-tumor,anti-aging,anti-radiation and so on.With the development of clinical research,ginseng polysaccharide is used more widely in the field of anti-tumor.Natural killer cells are important immune cells in the body,and they are the body's first line in defending against infection and tumor,they can directly kill the target cells,without antigen presentation.Ginseng polysaccharide has the functions of activating NK cells and promoting the killing activity of NK cells.It has been proved that ginseng polysaccharide has a certain effect on the activity of NK cells.In this study,we investigated the effects of ginseng polysaccharide on the killing activity of NK92-MI cells by detecting the surface activated receptors,the killing effect medium and profiling cytokine expressions in NK cells.Methods: NK92-MI cells were treated with ginseng polysaccharide(GPS)in concentration of 200mg/L,400mg/L respectively,and the control group was treated with PBS buffer.Using flow cytometry method to detect the levels of NKp30,NKp44,NKp46 and NKG2 D in the surface of NK cells.Using CCk8 method to detect the cytotoxicity of NK cells to K562 cells.Using Western-blot method to detect the expression of NK cell killing medium.Protein microarray technique was used to detect the expression of profiling cytokine in NK cells,and 80 kinds of cytokines were detected.Results: 1.Compared with the control group,GPS of 200mg/L and 400mg/L can enhance the killing activity of NK92-MI cells to some extent,in which GPS of 400mg/L can significantly enhance the cytotoxicity of NK92-MI cells(P<0.05).2.Compared with the control group,both GPS of 200mg/L and 400mg/L can significantly increase the expression of NKp30 and NKp44(P<0.05);GPS of 400mg/L can significantly increase the expression of NKp46(P<0.01);However,GPS of 200mg/L and 400mg/L have no difference in the expression of NKG2D(P > 0.05).3.Compared with the control group,GPS of 400mg/L can significantly increase the expression of perforin and granzyme Bin NK92-MI cells(P<0.01),but GPS of 200mg/L had no effcet in the expression of perforin and granzyme B(P > 0.05).4.Compared with the control group,GPS could promote the expression of IL-2,IL-8,CSF-2,CSF-3,MCP-1,MIG,IFN-?,and TNF-?in NK92-MI cells,in which GPS of 400mg/L can significantly enhance the expression of above profiling cytokine in NK92-MI cells(P<0.05).Conclusion: 1.GPS can promote the cytotoxicity of NK92-MI cells.The higher the concentration of GPS,the higher the cytotoxicity of NK92-MI cells have.2.GPS can enhance the killing activity of NK92-MI cells by increasing the expression of NKp30,NKp44 and NKp46 receptors.3.It has concentration dependence in expression of perforin and granzyme B by increasing GPS,only high concentrations(400mg/L)of GPS can increase the expression of perforin and granzyme B.4.GPS could promote the expression of IL-2,IL-8,CSF-2,CSF-3,MCP-1,MIG,IFN-?,and TNF-? in NK92-MI cells.