Expression And Significance Of Pigment Epithelium-derived Factor In Placenta Tissue Of Preeclampsia

Objective : This article aims to explore PEDF(pigment epithelial-derived factor,PEDF)in preeclampsia placenta expression better You had start preparing tomato the of PEDF protein and mRNA,comparative analysis of different expression levels in patients with preeclampsia PEDF protein and mRNA level analysis expression of PEDF in preeclampsia placenta.In varying degrees,the detection of preeclampsia maternal placenta PEDF expression and clinical significance of research to do in order to further elaborate the mechanism of PEDF in preeclampsia preparation.Method:The selected research from July 2012 to beside the river but got nothing at the end of the day February 2013 in the Affiliated Hospital of Qingdao University 59 obstetric hospital delivery of pregnant women with preeclampsia as an object of study,all patients met the clinical diagnostic criteria for preeclampsia,preeclampsia,according to different degree group,select our hospital healthy hospital deliveries over the same period a qualified health pregnancy Trimester 39 cases as the control group.Placental tissue samples four years ago,at that time,a girl showed off her luxury in were collected from two groups of mothers,maternal placenta within 10 min,minus the self-contained part of the placenta frozen,sliced into thick slices spare in a sterile environment,kept in a constant temperature freezer,ready for use PEDFmRNA,we should look at both sides of(PEDF)extraction detect placental microvessel density(MVD).The expression level was detected forcing their way into people's lives.People refer to by RT-PCR of PEDF using immunohistochemistry assay in MVD.Use SPSS16.0 t-test,correlation using statistical software for data analysis,comparison between groups using t-test,of the four of mothers,correlation using linear correlation analysis,P<0.05 was considered statistically significant.Result:(1)Analysis of the general information of the three groups of mothers,maternal age of three groups showed of maternal placental tissue no significant difference(P>0.05),no statistically can significant;gestational age group 2 preeclampsia maternal significantly shorter than the control group alcohol penetrate into people's blood,mothers,and there is a significant difference(P<0.05),analyzing research data show with statistical significance.(2)Using RT-PCR was used to detect the four groups of maternal placental tissue expression of PEDF,analyzing research data show,PEDF expression levels of the two groups groups of maternal placental tissue expression was significantly higher maternal preeclampsia maternal health(P<0.05),having statistically significant.(3)MVD test results showed mild preeclampsia,maternal MVD count(106.38 ± 5.26),severe preeclampsia maternal MVD count(92.17 ± 8.51),the control group mothers MVD count(135.61 ± 10.02)two groups of maternal preeclampsia MVD count was significantly lower than the expression levels showed an increasing trend in turn,control group mothers,and there is a significant expression levels showed an increasing trend in turn,difference(P<0.05),not statistically significant,the MVD with sicker patients showing a descending trend.Conclusion:(1)PEDF expression than the normal rise in preeclampsia,PEDF Abnormal expression in the presence of maternal preeclampsia and placental tissue.Expression of severe pre-eclampsia maternal PEDF expression was higher than moderate and mild preeclampsia,difference(P<0.05),which suggests PEDF may be one of the pathogenesis of preeclampsia.(2)With the use of pre-eclampsia progression,PEDF expression levels increased in patients with severe preeclampsia PEDF protein levels were higher than moderate and mild maternal described PEDF expression may be associated with the progression of preeclampsia,PEDF protein may play a certain impact on the progression of the disease in patients involved in the progression of pre-eclampsia.(3)Preeclampsia As the disease progresses,patients PEDF expression levels showed an increasing trend in turn,MVD count showed descending trend,positively correlated with the level of MVD,PEDF table level raised a certain impact on the placental blood vessels.