The Establishment Of Net Quality Standards For Silver Shavings And Observation Of Its Clinical Efficacy

Objective:To establish the quality standard of Yin Xiejing and combine NB-UVB to observe its clinical therapeutic effect.Methods:The main constituents were identified by TLC in Yin Xiejing,To verify the microorganism limited inspecting method of Yin Xiejing,the content emodin and chrysophanol in Yin Xiejing Tincture was determined by HPLC;60 patients with psoriasis vulgaris were randomly divided into treatment group and control group with 30 cases in each group.The two groups was given NB-UVB treatment.the treatment group on the basis of NB-UVB given Yin Xiejing treatment.The treatment course lasted for eight weeks.Results:TLC method for the identification of majorcomponents was specific.Membr ane filtration method is suitable for microbial limit test.The calibration curve of emodin was linear within the concentration range of 2.04?g/ml~24.48?g/ml(y = 44.737x-2.9878,R12 = 0.9995).The calibration curve of chrysophanol was linear within the concentration range of 2.04?g/ml~24.48?g/ml(y = 66.738x-12.416,R22 = 0.9993)The detection limits of emodin and chrysophanol were 0.041 g/ml and 0.025 g/ml respectively,The quantitative limits are 0.204?g/ml and 0.200?g/ml,respectively.The average recovery of emodin and chrysophanol was 95%~100% RSD=0.23%~1.15%(n=9)It shows that the content determination method has high recovery rate.The relative standard deviations of emodin and chrysophanol content were 0.64% and 0.88%,respectively,when the solution was placed at room temperature for 12 hours.It shows that the stability is good.The relative standard deviations of emodin and chrysophanol content were 0.63% and 0.45% after centrifugation and filtration treatment.The results showed that centrifugation and membrane filtration had no effect on the determination of the two components.The medium,low and high concentration emodin and chrysophanol were added to the blank adjuvant,and the average recovery was between 99% and 103%.RSD=0.83%~1.38%(n=9)It shows that the method is accurate.According to clinical observation,the total effective rate in treatment group was 90.0%,which was higher than 63.3% in control group,the difference was statistically significant(P<0.05).Conclusion: The determination method was proved to be simple,rapid and accurate.NB-UVB combined with Yin Xiejing Tincture,is a safe and effective choice for pasoriasis vulgaris.