Basic Research On Sensitizing Substances Of Shuanghuanglian Powder Injection

Shuanghuanglian injection has the dual role of anti-viral and antibiotic effective,but frequently reported adverse reactions,so studying the material basis of its adverse reactions must be resolved.In this paper,using partial least squares regression method HPLC characteristic spectral data associated with the efficacy data,research SHL injection anaphylactoid reactions spectrum effect relationship,initially clarify Shuanghuanglian sensitization component group.Main thesis is divided into two parts,the related research SHL injection of chemical constituents,pharmacological and clinical applications,adverse reactions and anaphylactoid reactions in the first part of the mechanism were reviewed;second part includes topics overall technology roadmap,SHL a series of experiments on chemical constituents of powder for the content,qualitative research Shuanghuanglian peaks characteristic patterns to create and efficacy studies class anaphylaxis,spectrum efficiency analysis,verification and efficacy of human serum components analysis.SHL injection adverse reactions in 75%of the department of allergy,and most occur for the first time,and there was no significant increase in IgE,namely class allergic reactions.Anaphylactoid reactions including direct mechanism of histamine release,complement activation,inhibition of the synthesis of eicosanoids abnormality like bradykinin,the activation of the coagulation system,kallikrein-kinin system,the role of platelet activation.Apart from the above reaction path,the application of irrational drug classes can cause allergic reactions,the majority of these reactions in a dose-dependent factor is allergen.Process of its occurrence mechanism of the antigen is sensitized mast cells,IgE antibodies on the surface of basophils in combination Fc?R bridged degranulation induced release of histamine and other inflammatory mediators,the inflammatory mediators acting on the respiratory mucosa can be manifested as symptoms of anaphylactic shock.SHL injection have been reported mainly in the chemical composition of flavonoids,lignans,phenolic acids,as well as other types of anthraquinone components.Pharmacological Effect of cocklebur focused in antibacterial and antiviral research.SHL injection with detoxification,dispelling wind and relieving for exogenous cold-induced fever,cough,sore throat;upper respiratory tract infection,pneumonia,lightweight,tonsillitis see the above symptoms.However,basic research is still in the preliminary stages of allergenic substances Shuanghuanglian of allergenic ingredients is not clear,largely affecting the quality control of SHL injection,the mechanism of action and clinical application,so a certain amount of research methods allergenic material basis to find Huang powder injection dual significance.The experiment system separation to give compound 11,wherein the compound 1,2,4,5,6,7 and 8 these seven compounds were obtained from the first compound,the raw material compound7and 8 of honeysuckle Shuanghuanglian,Scutellaria,Forsythia also been reported.Using partial least squares regression method,the SHL injection features 10 maps each batch HPLC data spectral efficiency and efficacy data correlation analysis,the independent variable X(peak area features on the map)on the dependent variable Y(histamine release)of the regression coefficients,as a percentage of positive regression coefficient associated with the plot of the corresponding peak area was occupied as sensitization and efficacy contribution rate forecasts which X12,X10,the compounds contribute to the efficacy rate of 90.21%.Learned from the fingerprint peak vest,X12,X10 respectively baicalin,rutin.Human serum combined pharmaceutical composition analysis,and found that baicalin was different.