Based On Data Mining, The Study Of The Prescription Of Cerebral Infarction Recovery Period And The Intervention Of Ditan Tongyu Recipe On IRAK-4 In Acute Hemorrhagic Stroke

ObjectivePurpose one Data mining based on the treatment of cerebral infarction convalesce nce of Chinese medicine prescription drugs,prescription law analysis of traditional Chin ese medicine in the treatment of cerebral infarction,provide data support and reference for the clinical reasonable medication.The prescription of enriching,and observe the w estern medicine in the cerebral infarction recovery association between the period and the TCM stroke.Purpose two Objective to investigate the effect of Ditan Tongyu decoction,on hep atic injury in rats with acute hemorrhagic stroke and the expression of interleukin-1 r eceptor related kinase-4(IRAK-4).MethodsMethod one Collect information about the clinical literature Chinese CNKI,Wanfan g database recovery stage of cerebral infarction.and using the V2.5 platform to establis h a database,prescription data analysis system "module application software",analysis of prescription frequency related database construction,analysis of prescription rule analysis and new prescription.Method two The healthy male adult SD rats by enzymatic digestion method,densit y gradient centrifugation and adherent KC extraction method,were randomly divided int onormal group,cerebral hemorrhage group,phlegm and blood stasis group,each group wa s divided into 6h,12h,24h,48h 4 phase sub group,IRAK-4 mRNA expression by rea ltime fluorescent fabric quantitative observation of liver PCR group,enzyme-linked imm unosorbent assay(ELISA)detected in the culture supernatant of TNF-a.Clinical Research Results(1)Clinical literature study screened in accordance with the standards of the 137 articles,220.220 get prescription prescription involves 225 flavors of Chinese medicine,the frequency in the top ten for chuanxiong,angelica,earthworm,leech,astragalus,Da nshen,Honghua,Shichangpu,peach kernel,radix paeoniae rubra,commonly used drugs co mbined with Rhizoma Chuanxiong,Angelica "","dragon",Rhizoma Chuanxiong,Rhizo ma Chuanxiong,Radix Astragali"," core drugs chuanxiong,angelica,earthworm and 1 eech,and the evolution of 16 core,get the new 12.(2)Experimental study of Ditan Phlegm Stasis Recipe can inhibit the expression of IRAK-4 and mRNA in liver KCs cells of rats with acute hemorrhagic stroke,and c an significantly reduce the content of TNF-?,and the expression of IRAK-4 protein in Chinese medicine group is positively related to the level of TNF-alpha of supernatant fluid.Research Conclusions(1)Conclusion cerebral infarction included clinical literature research China CNK I and Wanfang database on the recovery period of the use of drugs in the prescriptio n of Yiqi Huoxue Tongluo prescription is used.The Jieyu Dan,buyang huanwu decocti on,Tongqiaohuoxue decoction.(2)Experimental study on the conclusion IRAK-4 is an important regulator of L PS signal transduction,Ditan Tongyu decoction.The inhibition of IRAK-4 kinase activit y caused by downstream key inflammatory factor TNF-a generation were significantly reduced,therefore,phlegm and blood stasis activating side inhibition of KCs IRAK-4 is one of the important mechanisms of phlegm and blood stasis to relieve the liver dama ge.