The Effect Of Needle Knife Intervention On Oxidative Stress Injury In Parkinson's Model Rats

Objective:We investigated the effect of acupotomy on superoxide(SOD),glutathione peroxidase(GSH-Px)activity,contents of glutathione(GSH)and malondiadehyde(MDA)in corpus striatum and expression of tyrosine hydroxylase(TH)in Substantia Nigra of rats with Parkinson's disease(PD),to explore the mechanism of action of acupotomy on intreatment of PD.Methods:Ninety-six male Sprague-Dawley rats were randomized into a blank group,a sham group,a model group,an electroacupuncture(EA)group,an acupotomy group and a drug group,with sixteen rats in each group.Models were induced using an established two point stereotactic 6-hydroxydopamine(6-OHDA)injection in the right striatum and the apomorph-ine(APO)was used to induced rotation behavior.Both the blank group,a sham group and model group received normal feeding without any treatment.Rats in EA group were treated by EA at "Baihui"(GV 20)and "Taiyang"(EX-HN5)using the HANS LH202H EA device with dense-disperse wave(100Hz,1mA)for 20 min,3 times a week for 4weeks.Acupotomy group chose midpoint of the occipital bone inferior nuchal line and transverses process posterior tubercle C1 and C2 to debond and release,3 times a week for 4 weeks.The rats in the drug group were treated with the Levodopa(50mg/kg),once a day for 4 weeks after intervention,substantia nigra(SN)tissue was histologically and immunohistochemially examined.Additionally,SOD and GSH-Px activities,GSH and MDA levels were measured in striatum tissue.Results:1.The comparison of rotating behavior among each group show that the blank group and sham group had no rotation behavior(P>0.05).Before intervention,there was no significant difference between the model group,the acupotomy group,the EA group and the drug group(P>0.05).After intervention,no blank group and sham operation group have no significant difference in the number of revolutions(P>0.05).In acupotomy group,EA group and drug group rotations had decreased significantly after treatment(P<0.01).2.TH immunoreactive cells were observed that in the blank group and sham operation group have dense TH immunoreactive cells,and there was no difference(P>0.05).In the model group the neural cells and(TH)immunoreaction positive cells obviously reduced compared with the blank group(P<0.01).In acupotomy and EA group,the expression of(TH)immunoreaction positive cells increased significantly comperied with model group(P<0.05).The expression of TH immunoreactive cells in the drug group was significantly higher,but there was no differencecompare with model group(P>0.05).3.We observed that in the blank group and sham operation group have no difference(P>0.05).Following the intervention,we found that the decreased SOD and GSH-Px activity,and the increased GSH and MDA content levels of the striatum in model group,which have significant differences compaired with the blank group(P<0.01).After intervention,acupotomy obviously improve SOD and GSH-Px activities,GSH-Px levels and decrease MDA levels in the striatum of PD rats,showed significant difference comparing with the model group(P<0.05).EA and drug group could increase SOD activities and reduce MDA levels,showed significant difference comparing with the model group(P<0.05),and they promoted GSH-Px activities while showed no difference compared with the model group(P>0.05).EA also can improve GSH levels and show difference between the model group(P<0.05).Conclusion:We conclude that acupotomy can improve the phenotype of dopaminergic neurons,the number of neurons,and the expression of TH in rats with PD.Furtthermore,increased SOD and GSH-PX activity and decreased MDA levels improve antioxidant capacity and reduce free radicals.Therefore,acupotomy may decrease oxidative stress and improve dopaminergic neuron impairment in PD dopaminergic neuron impairment in PD.