Effect Of Phlegm And Blood Stasis On Serum Uric Acid And Related Enzyme Activities In Hyperuricemia Model Rats

As one of traditional acupuncture methods in Traditional Chinese Medicine,blood-prinking therapy refers to discharge certain amount of blood by pricking the blood vessels with a three-edged needle or other sharp instruments in order to treat illness,with the fun-ctions of invigorating the blood and opening the collaterals,removing blood stasis and gene-rating new blood,dispelling blockages and coag-ulations,and clearing heat while relieving toxins,etc.In clincal practice,the tutor of author has found that the bloodletting therapy can effectively treatment of hyperur i cemia,lower serum uric acid levels.In the early stage,we have carried out the clinical research on the treatment of hyperuricemia by bloodprinking therapy,on the basis of this,this study is to explore the curative effect and possible mecha-nism of the treat-ment of hyperuricemia by bloodprinking therapy.This subject adopts adenine and ethambutol combined with gastric lavage to establish hyperuricemia rat model,by obser-ving the influence of puncture pricking blood therapy on hyperuricemia rat model of uric acid,serum enzyme activity and liver and kidney function.ObjectiveRats with hyperuricemic were included by adenine and ethambutol hydrochlo-ride intraga-strically.After modeling successfully,rats in blood-pricking group were pricked on the super-ficial vein of Weizhong acupoint,Zusanli acupoint and Fenglong acupoint;allo-purinol group lavaged allopurinol according to the body weight of rats.After the end of treatment,to observe the effect of blood-pricking on rat serum uric acid(SUA),xanthine oxidase(XOD),adenosine deaminase(ADA),serum creat-inine(Cr),serum urea nitrogen(BUN),cerealthird transami-nase(ALT)and aspartate transaminase(AST).MethodsA total of 40 male SD rats were randomly divided into four groups that is,a normal group(10 rats),a model group(10 rats),a allopurinol group(10 rats),a group blood pricking group(10 rats).From the beginning of the experiment,the normal group was fed with normal saline 350 mg/(kg.d),the other groups were given intra-gastric administration of adenine 100 mg/(kg.d)and ethambutol 250 mg/(kg.d)according to the weight,1 times a day.After 4 weeks of successful modeling,the normal group was fed with normal saline 350 mg/(kg.d),the other groups were given intragastric administration of adenine 100 mg/(kg.d)and ethambutol 250 mg/(kg.d)according to the weight,3 times a week to the end of eighth weeks of treatment to stop,in order to prevent rats from self-healing.Fourth weeks after the success-ful mod-el,ats in blood-pricking group were pricked on the superficial vein of Weizhong acupoint,Zusanli acupoint and Fenglong acupoint,The amount of bleeding per 0.2-0.3ml;the allopurinol group lavaged allopurinol according to the body weight of rats,2 times a week until end of treatment for eighth weeks.After the treatment,10%chloral hydrate solution was injected into the abdominal cavity of anesthetized rats.The SUA,Cr,XOD,ADA were detected by enzyme colori-metric method,urease was detected by BUN?ALT and AST were detected by pyruvate oxidase method.Result1.Body mass,renal mass,renal index:ompared with the normal group,the body mass of the model group,allopurinol group,pricking blood group significantly decreased(P<0.05),the renal mass,renal index significantly increased(P<0.05);compared with the model group,the body mass,renal mass and renal index allopurinol group ignificantly increased(P<0.05),the renal mass and renal index of pricking blood group significantly decreased(P<0.05);compared with allopurinol group,the renal mass and renal index of pricking blood group significantly decreased(P<0.05).2.Serum SUA content:compared with the normal group,the model group SUA signifi-cantly increased(P<0.05),compared with the model group,the drug treatment group,the treatment group SUA significantly decreased(P<0.05).3.Serum XOD,ADA activity:compared with the normal group,model group,ADA and XOD activity were significantly increased(P<0.05),compared with the model group,allopurinol group XOD activity was significantly decreased(P<0.05),pricking blood group XOD,ADA activity was significantly decreased(P<0.05).4.Serum Cr,BUN content:compared with the normal group,model group,Cr content of BUN were significantly increased(P<0.05);compared with the model group,allopurinol group Cr,BUN content had no obvious change,pricking blood group Cr,BUN decreased(P<0.05).5.The changes of serum AST and ALT:compared with normal group;ALT,AST activity increased significantly(P<0.05),compared with the model group,allopurin-of group AST activity did not change significantly,ALT activity was significantly enhanced(P<0.05),pricking blood group ALT,AST activity was significantly decreased(P<0.05).Conclusion1.Prinking blood at the piont of Weizhong,Zusanli,Fenglong can reduce renal index,relive congestion and inflammatory reaction of the kidney of the model in hyperuricemia rats2.Prinking blood at the piont of Weizhong,Zusanli,Fenglong can inhibit the activity of XOD and ADA,reduce uric acid synthesis,reduce the level of SUA in the model in hyperuricemia rats3.Prinking blood at the piont of Weizhong,Zusanli,Fenglong can Reduce the content of Cr,BUN and ALT,to a certain extent,can reduce the damage of liver and kidney of uric acid,but also can avoid the use of uric acid to reduce the damage of liver and kidney in Western medicine.