| Purpose:Paeoniflorin is one of the main active ingredient and the material basis of traditional role by the side of Sini. By studying after giving Sini different compatibility groups pharmacokinetic parameters of paeoniflorin in rat plasma proven metabolic variation in animals, so as to explore Sini pharmacokinetics of the drug through the angle synergies compatibility between the group confirms its side compatibility is reasonable, provide an important basis for optimizing and guide future clinical dosing regimen.Material and method:Experimental animals: SD male rats. Drug and standards: Radix, white peony root, citrus aurantium, licorice Pieces, paeoniflorin standard. Reagents: methanol, acetonitrile, phosphate, acetate, 10% chloral hydrate, ultra-filtered water. Methods: Rats were orally administered whole side Sini decoction and decoction of seven different groups of compatibility, high performance liquid chromatography detection of paeoniflorin plasma concentration, gives the average plasma concentration of each group- time curve and calculation software and statistical software to calculate and analyze the pharmacokinetic parameters of each group by pharmacokinetic.Results: 1. The half-life Sini group for the group the longest, longer-acting and can be stably maintained paeoniflorin in rats plasma concentration, with the demolition of the other party compatibility group incomparable advantages.2. Chaishao group Cmax values less Sini group, and the length of the half-life in each group with the smallest difference Sini group, suggesting that a combination of compatibility Bupleurum and peony can be more stably maintained and Sini All parties fairly basic paeoniflorin blood concentration, is conducive to maintaining the efficacy of the original parties, reflecting the importance of synergies between the two groups prescription compatibility.3. Citrus aurantium peony itself alone with the compatibility and can prolong the half-life of paeoniflorin; and when it used with Bupleurum, peony rats in vivo effect of improving blood concentration glycosides role. This result suggests that the impact on citrus aurantium paeoniflorin plasma concentrations of no fixed trend, after drugs combined with different compositions will paeoniflorin prescription metabolic processes produce different effects, taking drugs and more drugs lead to synergies between effect.4. Licorice can accelerate the rate of metabolism of paeoniflorin, each containing licorice compatibility group was significantly shorter half-life, however, with the exception of T1 / 2 value Sini group is much higher than the other groups, we can see the whole side of Sini paeoniflorin half the prolonged action is generated after the compound compatibility, compatibility speaks Sini had a split between the other party groups do not have drug synergism, the more conducive paeoniflorin play efficacy.Conclusion:Chaihu, Zhishi, Gancao were played on the efficacy of paeoniflorin played a positive role in promoting, after all-party compatibility, 4-flavored drugs different from the various parties split compatibility group synergy. Four complementary herbal medicine can be more long maintained Fang main active ingredient paeoniflorin in relatively stable in vivo plasma concentration, continued to play its favorable efficacy and compatibility group compared with the other very significant advantages. Chaihu and Shaoyao Shugan blood, peony and citrus aurantium adjuvant co memorial to the throne qi, xiere broken knot of power, so that the drug played a spleen licorice and peony and shared in effect, four taste synergistically between drug effects, strong evidence of the party Jun, Chen, Zuo, Shi,compatibility exquisite structure, which fully reflects the classics side Sini on the prescription of scientific rationality. |
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