Studies On Chemical Constituents And Biological Activities Of Grasshoppers

Objective:To investigate components of Catharsius molossus.Methods: Separation and purification of the compounds was carried out by means of various chromatographic methods such as MCI gel CHP 20P,RP-18,Sephadex LH-20,PTLC,preparative and semi-preparative HPLC chromatography.The structures were identificated by using spectroscopic methods(MS,¹H NMR,¹³CNMR,2D NMR).Results: 41 compounds were isolated from the 70% ethanol extract of Catharsius molossus,they were identified as Molossusamide A?1?,Molossusamide B?2?,Molossusamide C?3?,cis-2-?3?,4?-Dihydroxyphenyl?-3-acetylamino-7-?N-acetyl-2??-amino-et hylene?-1,4-benzodioxane?4?,trans-2-?3?,4?-Dihydroxyphenyl?-3-acetylamino-7-?N-acetyl-2??-aminoethyl-ene?-1,4-benzodioxane?5?,trans-2-?3?,4?-Dihydro xyphenyl?-3-acetylamino-7-hydroxyethyl-1,4-benzodioxane?6?,trans-2-?3?,4?-Dihydroxyphenyl?-3-acetylamino-6-hydroxyethyl-1,4-benzodioxane?7?,tran s-2-?3?,4?-Dihydroxyphenyl?-3-acetylamino-6-?N-acetyl-1??-hydroxyl-2??-amino ethyl?-1,4-benzodioxane?8?,Aspongopusamide B?9?,trans-2-?3?,4?-Dihydr oxyphenyl?-3-acetylamino-6-?1??,2??-dihydroxyethyl?-1,4-benzodioxane?10?,Molossusamide D?11?,MolossusamideE?12?,p-Hydroxylacetonitrile?13?,N-?2-?3,4-Dihydroxyphenyl?-2-hydroxyethyl?acetamide?14?,N-Acetyldopa mine?15?,8-Amino-2?¹H?-quinolinone?16?,3,4-Dihydro-7-hydroxy-2?¹H?-quinolinone?17?,2-Piperidone?18?,cis-Cyclo?L-Pro-L-Tyr??19?,2?-?1?-Pyr azinyl?catechol?20?,3-Isopropyl-pyrrolopiperazine-2,5-dione?21?,Indolal?22?,Indolic?23?,?¹H-Indol-3-yl?oxoacetamide?24?,1,3-Dihydro-3,5-dihydroxy-3-methyl-2-indolinone?25?,3,4-Dihydro-1-?4-hydroxyphenyl?-¹H-2-benzopyran-6,7-diol?26?,Blapsin B?27?,7,4?-Homosisoflane?28?,Kaempferol?29?,?3R,4R?-3,4-Bis[?3-hydroxy phenyl?methyl]dihydro-2?3H?-furanone?30?,?E?-1,3-Bis?3,4-dihydroxyphenyl?prop-2-en-1-one?31?,7-Hydroxycoumarin?32?,3-Ethyl-6,8-dihydroxy-7-methyl-3,4-dihydroisochromen-1-one?33?,Monaschromone?34?,Methyl?E?-3-[?2R,3S?-2,3-dihydro-3-hydroxymethyl-7-phenyl)-1-benzo[b]furan-5-yl]-propenoate?35?,M-Hydroxyphenylacetic acid?36?,Protocatechualdehyde?37?,P-methoxybenzoic acid?38?,Vanillic acid?39?,3,4-Dihydroxy-benzalacetone?40?,and 3-Phenylpropionic Acid?41?,respectively.10 compounds were isolated from the 70% ethanol extract of the part of the water soluble components of Catharsius molossus,they were identified as Polyrhadopamine C?42?,Ginsenine?43?,Anoe ctochine?44?,trans-2-?3?,4?-Dihydroxyphenyl?-3-acetylamino-6-?N-acetyl-1??-hydroxyl-2??-aminoethyl?-1,4-benzodioxane?45?,trans-2-?3?,4?-Dihydroxyphe nyl?-3-acetylamino-7-?1??,2??-dihydroxyethyl?-1,4-benzodioxane?46?,2-Piperid one?47?,Cyclo?Phe-Gly??48?,cis-Cyclo?L-Leu-L-Pro??49?,3-Isopropyl-p yrrolopiperazine-2,5-dione?50?,and cis-Cyclo?L-Leu-L-Pro??51?,respectively.Conclusion: 41 compounds isolated in this species deals with dopamine dimers,dopaminederivatives,quinolines,piperidines,cyclic dipeptides,pyrazine,indoles,pyrans,flaconoids,furans,fcoumarins,xylogens and benzene derivatives,Compounds 25,26 are new natural products;compounds 11,12,20 are new compounds;compounds 1-4,6 have been published,and compound 4 possesses potoent anti-inflammatory activity toward COX-2.10 compounds isolated from water soluble partsare dopamine derivatives,dopamine dimers,alkaloids,piperidines and cyclic dipeptides.Among them,compound 42 is a rare dopbamine anoligue bearing a sulfur in the structure,which has been characterized by our group from blank ants.This compound was found to have potent NSCs proliferation promotion activity and antirheumatic property.The anti-inflammatory activity of compound 4 reveals that there might be more components with potential value in Catharsius molossus.