Studies On The Chemical Constituents And Antirheumatic Activity Of Dianbaizhu

This thesis consists of three parts together.Chapter1ReviewStudies on clinical applications, the chemcals and pharmacological activities of Dianbaizhu were reviewed in this chapter on the basis28references.Chapter2Studies on anti-rheumatism activity of DianbaizhuFor further development of Dianbaizhu, different sources of Dianbaizhu, different medicinal parts of G. leucocarpa var. crenulata and different extractions of G. leucocarpa var. cremtlata were studied. Mouse macrophage cell strain J774were used to study anti-rheumatism activity of them. NO and TNF-aAssay Kit were used to study anti-rheumatism activity. Experimental results showed that G.fragrantissima, G. semi-infera, G. trichoclada surpass others. Different medicinal parts, the underground parts has a better anti-rheumatic activity. In the different of the root from G. leucocarpa var. crenulata, n-butanol and water extracts were superior to other extracts.Chapter3Studies on the chemical constituents of DianbaizhuGaultheria trichoclada is one of the sources of Dianbaizhu. Compounds were separated and purified by various sorts of column chromatographic techniques. Their structures were identified on the basis of spectral methods:1HNMR13CNMR and so on. As a result,8compounds were isolated from G. trichoclada and8of compound structures were identified, including1methyl salicylate derivate,2flavonoids and their glycosides,3triterpenoids,1lignans,1olefin. All of them were isolated from G. trichoclada for the first time, And eucalyptin, ursolic acid lactone,(7S,8S)-4,9,9’-dihydroxy-3,3’-dimethoxy-7,8-dihydrobenzofuran-the l’-propylneolignan and (Z)-3-decene were obtained from genus Gaultheria for the first time.