Plasma MicroRNA Expression And Clinical Significance Of Papillary Thyroid Carcinoma

Background: Papillary thyroid carcinoma(PTC)accounted for about 90% of thyroid malignant tumor,is the fastest incidence endocrine system malignant tumors in recent years.PTC onset conceals,patients without specific symptoms.Mainly by such as clinical palpation,B ultrasound and nuclide imaging methods screening for PTC,but the diagnosis of these methods is still very limited.At present,the ultrasound guided fine needle puncture cytological biopsy(US-FNAB)is the most effective way of preoperative diagnosis in spection of thyroid nodule.But about 20% of thyroid nodules by US-FNAB is difficult to diagnosis,and belongs to the invasive examination,have certain risk,also need to consider the patient's compliance.Therefore,to obtain a new diagnosis method to improve the diagnosis efficiency of PTC have become the main direction,the researchers to explore with micro RNA(mi RNA)as a marker has become the study hotspot.micro RNA is a class of endogenous single non-coding small RNA molecules that length of about 22 nucleotides.The widespread distribution in eukaryotes.mi RNA with target messenger RNA(m RNA)the 3 'end of the translation section complete or incomplete complementary combination of degradation of the target or deter the messenger RNA transcription translation.The tissue differentiation,cell differentiation and apoptosis play a important role in regulating normal physiological process.Existing research shows that some of mi RNA in different diseases or pathological conditions change can be stable,specific reflections in the circulation of the blood.And can be tested to the amplification by molecular biology methods,providing new ideas for disease diagnosis and prognosis judgement.Along with the deepening of the research on mi RNA,the use of circulating mi RNA as diagnostic markers become research hotspot.Seeking potential diagnostic value of plasma inPTC specificity of mi RNA will fundamentally improve the diagnostic efficiency of PTC,offers a new approach for noninvasive diagnosis of PTC.Objectives: Quantitative real-time polymerase chain reaction(q RT-PCR)was used to detect mi RNA in the plasma and estimate their diagnostic usefulness for discriminating between benign and malignant lesions.The correlation of mi RNA change and the clinicopathological parameters of patients with PTC was analyzed.Methods: Patients who underwent thyroidectomy for benign thyroid nodules or PTC were enrolled in this study.The patients were divided into three groups: benign,PTC without lymph node metastasis(LNM),and PTC with LNM.The levels of mi R-146 b,mi R-221,mi R-222,and mi R-155 mi RNA expression in blood samples before surgery were evaluated.Results: Of 84 patients enrolled in this study,32 and 52 had benign lesions(38.1%)and PTC(61.9%),respectively.The mean levels of mi R-146 b and mi R-155 expression were higher in the PTC group than the benign group.For discrimination between benign and PTC lesions,the area under the ROC curve(AUC)for mi R-146 b was 0.649 with 61.4% sensitivity and 57.9% specificity.The AUC for mi R-155 was 0.695 with 74.3% sensitivity and 63.2% specificity(P < 0.05).The levels of mi R-146 b,mi R-221 and mi R-222 were slightly higher in the N1 group than the N0 group.The levels of mi R-146b?mi R-155 and mi R-222 increased in proportion to tumor size.Conclusions: mi R-146 b and mi R-155 helped to discriminate between benign and malignant lesions.Circulating mi RNA is likely an useful alternate serological marker for PTC.This preliminary study suggested that circulating mi RNA may be useful as follow-up tools as well as diagnostic tools.