The Effect Of Rainfall Enhancement On The Genetic Diversity Of Brandt's Voles

Lasperodomys brandtii is one of the typical grassland rodents in China,which seriously damaged the grassland vegetation and affected the production of agriculture and animal husbandry in China.The study of the population fluctuation mechanism can effectively prevent and control the occurrence of rodents,and with the global climate changes,environmental factors will affect the number of animal populations,which rainfall is the number of animal populations affect one of the main environmental factors.The genetic diversity of animal species in the animal population fluctuations play an important role,rainfall of animal population genetic structure of the control effect is not clear.In order to explore the effect of precipitation on the dynamics of animal population,this study is based on the large-scale controllable fence of Inner Mongolia grassland,the microsatellite markers were selected from the populations of Lasiopodomys brandtii,and the effects of artificial rainfall treatment on the genetic diversity of Brandt ’s voles were examined.Through to the precipitation conditions of Brandt’s vole allele number(Na),effective number of alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He),polymorphism information content(PIC)analysis,study population genetic diversity of Brandt’s vole rainfall regulation effect,thus to provide theoretical basis for reasonable control rat.The results show that:(1)The average number of alleles in the control group was 4.90,the average number of alleles in the light-rainfall-treated group was 7.80,the average number of alleles in the moderate-rainfall-treated group was 4.60,There was significant difference between rain treatment and control group(P<0.05);(2)The average number of effective alleles in the control group was 3.65,the average number of effective alleles in the light-rainfall-treated group group was 4.02,and the average number of effective alleles in the moderate-rainfall-treated group was 3.32,The difference was not significant(P>0.05);(3)The average observed heterozygosity of the control group was 0.38,the average observed heterozygosity was 0.63 in the light-rainfall-treated group,the average observed heterozygosity was 0.44 in the moderate-rainfall-treated group,There was significant difference between rain treatment and control group(P<0.05);(4)The average expected heterozygosity of the control group was 0.68,the average expected heterozygosity of the light-rainfall-treated group was 0.69,the average expected heterozygosity of the moderate-rainfall-treated group was 0.66,The difference was not significant(P>0.05);(5)The average content of polymorphic information in the control group was 0.69,the mean content of the polymorphic information was 0.64 in the light-rainfall-treated group,and the average content of polymorphism information in the moderate-rainfall-treated group was 0.61,The difference was not significant(P>0.05);(6)Under the condition of the light precipitation enhancement,the average number of alleles in female Brandt’s vole is 6.40,the average effective number of alleles is 3.77,average observed heterozygosity was 0.58,the average expected heterozygosity was 0.68,the average polymorphism information content was 0.64;Under the condition of moderate precipitation enhancement,the average number of alleles in female Brandt’s vole is 3.30,the average effective number of alleles is 2.59,average observed heterozygosity was 0.39,the average expected heterozygosity was 0.55,the average polymorphism information content was 0.48;Significant difference was found in the female population genetic diversity under different processing(P<0.05);(7)Under the condition of the light precipitation enhancement,the average number of alleles in male Brandt’s vole is 6.30,the average effective number of alleles is 3.79,average observed heterozygosity was 0.69,the average expected heterozygosity was 0.71,the average polymorphism information content was 0.61;Under the condition of moderate precipitation enhancement,the average number of alleles in male Brandt’s vole is 4.60,the average effective number of alleles is 3.55,average observed heterozygosity was 0.69,the average expected heterozygosity was 0.70,the average polymorphism information content was 0.63;Under the different processing male population genetic diversity difference was not significant(P>0.05);.Through the experimental group and control group fence Brandt’s vole are significant differences between the population genetic diversity,the results showed the light precipitation enhancement processing fence Brandt’s vole species have higher genetic diversity and light precipitation enhancement processing of the female population genetic diversity is significantly higher than moderate precipitation enhancement processing of the female population.In addition,the above results are of great guiding significance for the reasonable control of the population of Brandt’s vole.