Research And Design Of Hand-held Tumor Fluorescent Marker Rapid Detection Equipment

Early detection,diagnosis and treatment of malignant tumors can effectively reduce lethality of tumor.Tumor marker is a kind of biomolecules mainly present in the blood.We also can get the changes by analyzing fluorescence intensity if we connect the marker with fluorescent substances.Although the existing immunofluorescence detector has a certain degree of detection accuracy,the acquisition and analysis of fluorescence signal is mainly realized by the hardware circuit,which limits the size and power consumption of the detector.What’s more,they are only suitable for medical institutions such as hospitals,not suitable for self-testing simple and quick in the home or community due to its expensive price.In view of above situation,a kind of portable low-power tumor marker fluorescence detection equipment has been designed in this paper by analyzing the corresponding relationship between the fluorescence intensity and the concentration of the fluorescent substance.The upconversion luminescent material is used in this device as a fluorescent label based on the study and analysis of existing fluorescent markers.Upconversion material is excited by near-infrared light at 808nm and emits green fluorescence at 560nm,which has advantages such as narrow emission band,small background fluorescence,high quality light stability and small biological toxicity.The device uses low-power optical components and electronic components for portable design requirements.Taking into account of the software part of the fluorescence detection equipment in the basis of ensuring the basic functions of the hardware part.Digital phase-locked algorithm(DPLA)and shaped function are applied to the initial acquisition of fluorescence signal processing equipment with weak signal detection as a significant feature.Shaped function can extract weak signals that are lower than quantization step effectively.Digital phase-locked algorithm can eliminate effects of noise in weak fluorescent signals.What’s more,optical shaped function is used to replace traditional electrical shaped function,which can eliminate the effects of sensor thresholds on the results effectively.4 times of modulation signal frequency are used as sampling in the digital phase-locked algorithm.Compared to traditional digital phase-locked algorithm,the number of operations have been greatly simplified,which effectively improve the running speed.Retaining its detection accuracy and reduce system power consumption as well as volume.In this paper,control standard is 7-FRSpec fluorescence spectrometer.Design and implementation of fluorescence detection equipment includes four parts:detection principle,optical structure,hardware and software algorithms.Advantages of the fusion algorithm under weak fluorescence signal detection have been shown compared with detection results without the algorithm.Overall performance test of the prototype equipment also has been completed in the end.Working time of the whole machine is more than 8 hours,which meets the design index.The calibration factor for the linearity test is 0.982.The coefficient variation of the fusion algorithm is 0.45%,indicating that the system has good linear response characteristics and data stability.The test results show that the design can achieve the desired effect,which also contain a more accurate measurement and display of solution concentration.