Preparation And Application Of Fluorescently Imprinted Nanomaterials For Detecting Glycoproteins

Glycoprotein is an important macromolecule in the organism,which is the product of glycosylation of protein and plays an important role in the organism.Studies show that the levels of glycoprotein expression in the body are closely related with variety kinds of diseases.For example,membrane-bound glycoprotein transferrin receptor,the main role of which is to transport the iron to the cell via transferrin,will excessively express to meet the excess iron required for malignant proliferation of tumor cells when cancer occurs in the organism.And another membrane-bound glycoprotein mucin 1,the main role of which is to maintain the pipeline lubrication,adhesion of the luminal surface of the bacterial virus under normal conditions will excessively express significantly when cancer occurs.Therefore,developing new methods with good stability,simple operation,low cost and rapid detections of glycoprotein are important for studying the development of related diseases.We designed and synthesized two fluorescent nano-materials based on molecular imprinting,which can detect and specifically identify the transferrin,transferrin receptor and mucin 1 and applied for recognition of the template in cell and in vivo.1.The fluorescence protein imprinted polymer were prepared using the silica coated Rhodamine B as the core,modifing the boric acid on the surface by sol-gel and horseradish peroxidase as template.In order to verify the universality of the method,another protein transferrin was used as the template molecule to prepare molecularly imprinted polymer.The fluorescence response of the imprinted material to the template molecular transferrin is investigated.Finally,the specific identification and fluorescence imaging research of the transferrin receptor on membrane is carried out by transferrin as mediator.The fluorescence material has the advantages of simple operation and fast identification which achieves selective identification of transferrin receptor-related circulating tumor cells through the specific identification of transferrin.This work provides a new idea for researchers to design synthetic artificial antibodies.2.The boric acid groups are modified on the surface of the upconversion nanoparticles.The mucin1 was used as template to prepare the molecularly imprinted polymer.The recognition performance of the material is investigated by fluorescence response and the material is applied to the specific identification and image research of the micin1 on membrane.Finally,it was applied to the treating of mouse breast cancer model,and tumor cells are killed through the orientation of the imprinting material and the thermal effect under 980 nm laser irradiation.The experimental process and the results show that the imprinting polymer based on upconversion nanoparticles has the advantages of small background interference,simple operation,and the realization of tumor recognition and treatment integration.This method is instructive for the clinical study of the diagnosis and treatment of cancer.