Preparation And Application Of Nanofiber Artificial Extracellular Matrix

In this paper,Zein/Rat tail collagen I nanofibrous membrane was prepared by electrospinning in order to mimic the natural extracellular matrix.Firstly,Zein/Collagen I composite nanofiber membranes were successfully prepared by electrospinning.The optimum spinning conditions were as follows:the concentration of zein was 30 wt%,the amount of collagen I was 26.7 w%,the spinning voltage 19 kV,0.8 mL/h of spinning flow and 15 cm of receiving distance.The FTIR spectra showed that the composite fiber membranes were composed of zein and collagen I.Zein nanofiber membrane and composite membranes had a specific surface area of 9-10 times that of Zein blend membrane.The results of water contact angle and water absorption test showed that collagen I could effectively improve the hydrophobicity of zein fiber membrane and enhance the performance of membranes absorption liquid.The mechanical property test showed that the fiber membrane had good flexibility The stability of the membrane in water and enzymatic degradation experiments showed that the membrane could be stable in the solution environment for a short time,and the fiber membrane degraded slowly with time,laying the foundation for the next step as drug delivery carrier.Secondly,the biocompatibility of Zein/Collagen I nanofibrous membrane was studied.The results of cell adhesion test showed that L929 cells adhered to the membrane surface within 2 h and the cell adherence rate was higher than that of the control group L929 cells adhered,expanded and proliferated on the surface of the fibroblasts by MTT assay and electron microscopy.The results showed that L929 cells could maintain normal growth state,and the cell adhesion rate increased with the increase of collagen I content.Prolonged,the number of cell proliferation gradually increased.Finally,Zein/Col/Drug nanofiber membranes were successfully prepared and the controlled release of naproxen sodium was studied.The other spinning parameters were the same,the optimal drug concentration was 2 wt%.The results of FTIR and TEM showed that the fiber surface was smooth and the drug molecules were evenly distributed in the composite fiber.The pH value of the dissolution solution was within the scope of the medical device,and the antibacterial test showed that the antibacterial property of the fiber membrane was obvious and effective The inhibition of bacterial activity;the medical gauze as a control,through the whole blood clotting and plateletadhesion experiments not only shows that drug-loaded fiber membrane has a good blood clotting effect,the higher the collagen content,the better the blood clotting effect;and can activate platelet activation,the content of platelet adhesion increased.The drug-loaded fibrous membrane could slow down the release of the drug with the gradual degradation of the fiber membrane in the 37 ? PBS solution,thus avoiding the phenomenon of drug burst release.