Research On The Construction Of Plasmid Components Based On Biological Big Data Using Ordered Logic Gates

Rapid developments have been made in synthetic biology within the past two decades,particularly in combination with chemistry,physics,information science,engineering science,computer science,and other disciplines.Genetic parts and internal features have been a main focus of research for synthetic biologists,and the sequence information is the basis of genetic parts and internal features.Existing parts and internal features were often used before,the information are not necessarily comprehensive,accurate or easily accessible.In addition,sequence descriptions and formats are not always uniform and existing analytical methods are often time-consuming,thus complicating research on biologically relevant features is difficult to synthetic biologists.Based on the situation above,we analyzed large-scale and widely used data which containing over 70,000 feature sequences and over 7000parts.Using the delete statement in Structured Query Language,we obtained comprehensive non-redundant information for a complete data set.Contrast analysis of internal feature sequences of gene parts will provide synthetic biologists with the theoretical basis for constructing new plasmid parts with specific functions.GenoCAD is a computer-aided design software program for synthetic biology that can be used to design complex structures.We can design grammars and rules according to individual demand and design a synthetic DNA molecule.Logic gates can be applied in various disciplines,but have not yet been used for plasmid design,this paper puts forward an order logic gate which can building a biological part in greatly different from the past.We summarized previous related work of home and abroad synthetic biologists and put forward a new model.In this paper,the specific work is as followings:(1)In this study,we analysed a large,commonly used data set containing over 70,000 feature sequences and eventually obtained comprehensive information for a complete data set without redundancy.By analysing the internal feature sequences,we input the most representative data in the GenoCAD platform.(2)We design rules and grammar in GenoCAD,and then we use the entried sequence according to the actual demand to construct high-quality practical parts.(3)After analysis the structure of biological pars we put forward a new logic gate namely orderly logic gate,on the basis of the logic gate,we can carry out the study of parts internal features and tectonic sequence,which be useful for professionals and nonprofessionals and may have applications in the design of a new biological computer.(4)Finally,we compared the constructed plasmid with other successful examples in BLAST and PlasMapper software to demonstrate the rationality of the orderly logic gate.This paper design orderly logic gate model not only has simple structure,but also easy-operational and versatile,the design results facilitate testing.Finally,we through the computer software for sequence alignment,than the rationality of the model is proved by the results,it provides a theoretical basis for plasmid design.