Screening, Optimized Culture And Enzymatic Properties Of A Carrageenase-producing Strain Of Alteromonas Sp. R11-5

In this paper,12 strains with carrageenan activity were screened from 85 strains of Antarctic bacteria,and the highly active strain R11-5 was identified and optimized for its fermentation.The whole genome was sequenced and sequenced.Carla gelatin gene Car1853,and its efficient expression;purification of recombinant carrageenan enzyme,the system of its enzymatic properties,analysis of the degradation characteristics of the enzyme,to clarify its degradation products and their type,with a period of carrageenan and carrageenan Sugar industrial production to provide theoretical and technical support.In this experiment,the newly identified high activity carrageenan strain R11-5 was identified,and the results of morphological and 16 S rDNA species showed that the Antarctic bacteria belonged to Alteromonas.The optimum culture conditions were as follows: temperature 15.7?,pH7.0,yeast extract 0.6%,beef extract 1.17%,carrageenan 1.05 ‰,CaCl2 5.78 mmol/L,The maximum enzyme activity was 56.972 U · mL-1,and the activity of enzyme was 1.7 times higher than that of the control group.The recombinant plasmid pET30a-Car1853 was constructed by gene engineering,and the recombinant plasmid pET30a-Car1853 was transformed into Escherichia coli BL-21.The recombinant plasmid pET30a-Car1853 was constructed by gene engineering.SDS-PAGE showed that the molecular weight was 42 kDa.The results showed that the optimum reaction temperature was 55 ?,the optimum pH was 7.0.The ion has an inhibitory effect on its activity.Thin layer chromatography showed that the final product of carrageenan cleavage of carrageenan was mainly carrageenan.