Screening And Performance Study Of High-efficiency Chicken Manure Fermentation Bacteria

With the development of large-scale aquaculture enterprises,the production of a large amount of chicken manure has made it difficult to evacuate in a short period of time and seriously pollute the environment.Chicken manure fermentation is an effective treatment method to realize the harmlessness and resource utilization of chicken manure.It plays an important role in the treatment of livestock manure.Due to the insufficient amount of protein-degrading bacteria and high-temperature bacteria,the external environment,the current chicken manure fermentation time Long,it is difficult to meet the requirements of enterprises.The aim of this study was to screen high-efficiency high-temperature protein degradation strains by enrichment and pure culture,optimize the degradation conditions of protein-degrading bacteria,prepare high-quality microbial agents for fermentation application experiments,and study the preservation methods of microbial agents.Conclusions are as follows:(1)Under medium,high temperature and high aeration conditions,the organic nitrogen solution in chicken feed was used as the sole carbon source to enrich and culture the complex bacteria.Under the condition of medium temperature,the protein degradation rate of the complex liquid could reach 2500mg/L/d.The Kjeldahl nitrogen degradation rate can reach 3.2mg/m L/d,and the degradation cycle is 3 days.Using high-throughput sequencing technology,it was found that the main dominant fungi in the 40,50,and 60?enriched cultures were Deinococci,Gammaproteobacteria,and Bacillus.Gemmatimonadetes;The microbial community richness and uniformity of the composite bacteria cultured at 60? were relatively low;A total of 27 dominant species were detected in the enriched cultured mixed liquid samples at three temperatures.Truepera radiovictrix was a highly efficient protein-degrading strain with a relative abundance of 41.96% at 40?.Thauera linaloolentis Pseudoxanthomonas taiwanensis and Bacillus thermophilus can degrade proteins.The relative abundances in the composite bacteria samples cultured at 50 ?are 40.34%,10.48% and 6.11%,respectively.The relative relatives of Luteimonas compost in 60 ? cultured composite liquid samples,The abundance is 60.92% with protein degradation.(2)Total of 24 strains of protein-degrading bacteria were isolated and purified under high temperature and high aeration conditions.They were identified by Brevibacillus spp,Caenimicrobium spp,Bacillus spp,Pseudoxanthomonas spp,Chelativorans spp,Chelatococcus spp.Luteimonas spp and Bordetella spp.Among them,the protein degradation rate of strains No.1 and No.24 was 4800 mg/L/d.Optimize the degradation conditions of its highly degraded proteins.The ratio of carbon to nitrogen has a great influence on the complex bacteria of protein-degrading bacteria.When the ratio of carbon to nitrogen is 8:1,the protein-degrading strain can pass the lag phase and the log phase more quickly.For a single strain,small-molecule glucose As an external carbon source,the protein degradation rate has the greatest influence.The protein degradation rate of Brevibacillus borstelensis can be increased by 31.31%.When the concentration of glucose is 1.0g/L,the protein degradation strain can pass the lag phase and the log phase to reach stability more quickly.The trace elements have little effect on the degradation of protein degradation strains.The trace elements with 60?140?L can promote the degradation of protein degradation strains.The degradation effect of protein degradation strains beyond this concentration will be inhibited.(3)Preservation experiments on protein degradation strains.The survival rate and activity of the glycerol frozen broth preservation method were relatively high at-80 °C,followed by the preparation of the bacterial suspension at 4 °C for recovery of the preservation activity,and the rate of degradation of the protein in the second generation.Complete recovery,cryopreservation is beneficial to the growth activity of protein-degrading strains and the maintenance of metabolic activity.(4)The high-efficiency protein degradation strain was returned to the aerobic fermentation experiment.The odor of the experimental group after the addition of the prepared composite microbial agent disappeared completely,and the texture was soft and loose,with a strong acid flavor.In the fermentation process,the temperature of the four groups of fermentation materials reached 40? within 8 h,and the temperature of the single-serum composite bacteria group was up to 47?,and the temperature reached 60 ? at 48 h;The total organic carbon content of the four experimental groups decreased,and the total phosphorus,total potassium and total nitrogen increased.At 72 h,the moisture content of the four experimental groups was 24.69% and 19.97%,respectively.21.26% and 36.58%;compared with the blank control group,the inoculation of the fermenting agent is beneficial to the composting of the compost and the mutual transformation between various substances.The dominant bacteria in the high temperature stage of the four experimental groups were Firmicutes and Proteobacteria.The dominant species is mainly composed of Bacilli and Gammaproteobacteria.The fermentation bacteria added in this experiment accounted for a large proportion,and the total proportion of FJ1,FJ2 and FJ3 experimental groups were 54.18%,58.30% and 29.75%,respectively.Only Chelativorans composti was found in the blank group,accounting for 1.16%.This can verify that the microbial agent added in this experiment has a significant promoting effect on chicken manure fermentation.