Study On The Application Of Surface Enhanced Raman Spectroscopy And Surface Enhanced Fluorescence For The Fluorescein Detection In Microfluidic Chip

With the improvement of the technology level,the microfluidic chip has been paid more and more attention as an experimental platform.The surface enhanced Raman scattering technique is a powerful analysis tool for the narrow width of the spectral line.It can be used to identify and detect of low concentration substances which has gradually become a research hotspot in the field of biological detection.In recent years,the surface enhanced fluorescence gradually rise,when the fluorescent molecules located near the metal such as gold or silver nano structure or the sol,the fluorescence intensity of free fluorescence intensity be increased significantly,which provides a new possibility to further improve the detection limit of fluorescence detection and sensitivity,so that the surface enhanced fluorescence technology become a powerful tool used in surface science research,ultra sensitive biological detection as surface enhanced Raman scattering technique,Combined with the advantages of SERS and SEF,microfluidic chip based on SERS and SEF detection is of great significance for the acquisition and analysis of high sensitivity.This paper firstly introduces the need for SERS and SEF detection of silver nano materials and fluorescein SYBR Green I,and introduces the basic principle of Raman spectroscopy,fluorescence spectroscopy,the surface enhanced Raman scattering spectroscopy,surface enhanced fluorescence spectrum and the scattering mechanism should be used,and analyzes the advantages and disadvantages and the application prospect of two the.On this basis,the characteristics and research status of surface enhanced Raman spectroscopy(SERS)and the surface enhanced fluorescence spectroscopy in microfluidic chip are introduced.The main work and conclusions of this paper are as follows:(1)Studied the detection of fluorescein SYBR Green I by surface enhanced Raman spectroscopy in microfluidic chip.In the experiment,microfluidic chips produced by polymethyl methacrylate(PMMA).As SERS substrate,silver nanoparticles were produced by chemical synthesis.Compared and analyzed Raman spectra of SYBR Green I and SYBR Green I on SERS substrate of microfluidic chip.Different concentrations of SYBR Green I prepared to evaluate the sensitivity of the system.We found that the intensity of SERS signal intensity increased linearly with the concentration of SYBR Green I,results show that can use the system by measuring the intensity of the signal to detect the quantitiy of SYBR Green I.This method can be used to improve the quality of detection of SYBR Green I in SERS,and can also be used to improve the detection of other materials.(2)Few studies have been conducted to investigate surface enhanced Raman spectroscopy and surface enhanced fluorescence effects in microfluidic chips qualitatively and quantitatively.Based on the research of using surface enhanced Raman spectra to detect SYBR Green I in microfluidic chips,has also been studied using surface enhanced fluorescein to detect SYBR Green I,comparative analysis of the fluorescence intensity of SYBR Green I when the silver nanoparticles were added and not added in microfluidic chip,and study the effect of different concentration of SYBR Green I on fluorescence intensity in microfluidic chip.According to the results,it is concluded that the addition of silver nanomaterial can enhance the SEF signal greatly.This method can be used to improve the detection of SYBR Green I in SEF,and can also be used to improve the detection of other material.According to the above research shows that adding nano silver in microfluidic chip can improve the detection quality of SYBR Green I in SERS and SEF.Both surface enhanced Raman spectroscopy and surface enhanced fluorescence technique can be used for the detection of SYBR Green I.This technology has a good prospect in the fields of environment,chemical detection,biological and drug detection.