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Design And Application Of A New Type Of Sensor Based On Gold Nano Structure

Posted on:2017-04-19Degree:MasterType:Thesis
Country:ChinaCandidate:Q MaFull Text:PDF
GTID:2348330563450644Subject:Chemical engineering
Abstract/Summary:PDF Full Text Request
Nobel metal nanomaterials display unique optical,electrical,mechanical and catalytic properties due to the excellent effect of the surface,size and dielectric confinement.They have been widely used in the chemical analysis,immunoassay,biological sensing and so on.Gold nanomaterials were used widely in various fields because of its good biocompatibility and stability.In this paper,we develop the surface enhanced Raman scattering(SERS)active sensor and a new surface plasmon resonance(SPR)sensor chip based on gold nanostructure substrate for the ultra-sensitivity detection for persistent environmental pollutants pentachlorophenol(PCP)and lipopolysaccharide(LPS).Firstly,SERS substrates were prepared with gold nanoparticles through covalent bond using internal standard method for PCP detection in ultrapure water and tap water with 0.26 ?g/L measuring limit.There is good linear relationship between the ratio of internal standard SERS and the Log value of the PCP concentration with 0.9815 correlation coefficient during 0-100 ?M.The results show that the sensor has good selectivity,stability and re-useful ability.The detection limit is lower than the biggest pollution concentration of PCP defined by the U.S environmental protection agency.Secondly,a LPS recognition SPR sensor chip was prepared on the gold film surface using a specific sequence of peptides.In ultrapure water and serum resolution,there is a good linear relationship between SPR intensity and the log valves of the PCP concentration from 1.0×10-14 to 1.0×10-11 mol/L with over 0.99 linear correlation coefficient and.The detection limit is as low as 1.0×10-14 mol/L with good selectivity and well application possibility.
Keywords/Search Tags:gold nanoparticles, surface-enhanced Raman scatteringering, surface plasmon resonance, pentachlorophenol, lipopolysaccharide
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