Research On Deep Penetration Imaging Technology Based On Schwartz Aperture And Parallel Wavefront Optimization

Fluorescence microscopy imaging technology is widely used in biological and medical observation because of its biospecific labeling,high resolution,real-time imaging,sample loss,easy operation and so on.The most representative of which is the confocal microscopy system.A small hole is used to filter out the background signal by physical means to realize the three-dimensional reconstruction of the superficial surface of biological tissue with diffraction resolution.However,with the increase of the imaging depth,the optical error and scattering caused by the sample will increase sharply,and the background signal filtering ability of the small hole will be seriously weakened,which will make the resolution limit of the confocal microscope diffraction difficult to maintain.Based on the analysis of the principle of confocal microscopy,this paper proposes a variety of effective ways to further optimize and improve its background suppression ability,and further improve the imaging depth and system resolution.In this paper,we first introduce a confocal microscopy system,a deep penetration imaging method based on a confocal system,and an adaptive correction algorithm based on parallel wavefront optimization.In the following,the imaging performance of the confocal system based on the new Schwartz aperture is explored,and its signal intensity is more rapid than the confocal system in the axial direction.This feature facilitates deep imaging of the tissue.Weakened the interference from the integral signal on the focal plane,and the signal-to-noise ratio is improved.Then,the multi-perturbation compensation technique based on parallel wavefront optimization is studied.For the first time,the selection of two-photon modulation frequency is analyzed in detail,and an algorithm for generating two-photon multi-perturbation modulation frequency is proposed.Then the modulation frequency of the algorithm is verified,and the imaging performance of the two-photon parallel wavefront optimization system is further analyzed.Because the two-photon modulation frequency is more difficult than that of one-photon system,the alternative method of two-photon parallel wavefront algorithm is explored,and the feasibility and application condition of the alternative scheme are discussed.Finally,under the influence of noise,the correction performance of wavefront optimization technique is discussed.Finally,this paper summarizes the research work of this subject,and points out the next research direction.