Near-infrared Fluorescence Microscopy Applied For Study Of In Vivo Deep-tissue Imaging

Deep optical imaging of biology provides rich information for medical diagnosis and scientific research,in this filed,bio-fluorescence imaging technology occupies a very important part.In this article,the deep fluorescence microimaging studies of biological tissue were carried out with a clinical near-infrared fluorescence dye and another aggregation-induced emission(AIE)dye as fluorescence probe.We mainly introduced and explored the near-infrared laser scanning confocal microscopic imaging,two-photon fluorescence microscopic imaging and short-wave infrared fluorescence imaging,which can be concluded in the following content:We built a stable near-infrared laser scanning confocal microscopic imaging system,and adopt the near-infrared laser as excitation and near-infrared fluorescence as signal detected,realized deep brain blood vessels microscopic imaging of living mice,which reached imaging depth of 750 ?m.In order to further improve the fluorescence imaging depth of biological tissue,we built a two-photon excited near-infrared fluorescence microscopic imaging system.With the assistance of near-infrared two-photon fluorescent probes,excitation of 1040-nm femtosecond pulse laser and detection of near-infrared fluorescence,we realized deeper brain blood vessels microscopic imaging of living mice(950 ?m).In order to obtain deeper imaging depth and more abundant imaging information,we also explored the short-wave infrared fluorescence imaging.We built a short-wave infrared fluorescence in vivo imaging system.With the asistance of short-wave infrared fluorescent probes,635-nm excitation with low power density and short-wave infrared camera with high sensitivity,we realized short-wave infrared angiography of blood vesseles in whole body and brain of living mice.On this basis,we also set up a new short-wave infrared fluorescence microscopic imaging system.With the assistance of short-wave infrared fluorescent probes and short-wave infrared camera with high sensitivity,brain blood vessels microscopic imaging of living mice with greate depth(800 ?m),high spartial resolution(<3 ?m)and high temporal resolution(25 Hz)was realized.Furthermore,with the assitance of the short-wave infrared fluorescence microscopic imaging system,we also realized the functional imaging of detection of the blood-flow speed in living mouse brain vascular,the detection of thrombosis in living mice brain and the detection of EPR effect in tumor site.