Plants-bionic Integrated Cell Manipulation Of A Microfluidic Chip

The traditional cell culture environment is very difficult to close to cells in vivo microenvironment,it is hard to get more fit treatment of the body.The emergence of microfluidic chip for cell research provides a new opportunity,the design of micro channel/microstructure provides cells with relatively close to the human body cell growth environment,but the fluid in the microchannel/microstructure has some influence on cell growth and stability of the micro environment for cell growth is still we need to solve the problem.This paper has designed and produced a kind of bionic integrated cell manipulation of the microfluidic chip which can provide stable microenvironment,and conducted the following research:(1)Study of dicotyledonous plants reticulate venation and moisture transport properties.Dicotyledonous plants with reticulate venation structure,it has strict layer and distribution characteristics,and follow the laws of Murry.Leaf veins distribution tree bifurcation structures step by step,not only as the blade supporting structure also provides the leaves in the water transport multiple channels.Surrounded by many fine veins form a mesh structure,mesophyll cells in here growth,water and inorganic salt and other nutrients through the pit to the mesh hole for the growth of mesophyll cells.Reticulate venation for the growth of leaf mesophyll cells within a stable growth environment.(2)Structure design and fabrication of biomimetic chip.In this paper,based on the characteristics of dicotyledonous plants reticulate venation,designed a series of cell culture with mesh structure of microfluidic chip.Sample chips made from three layers of structure: cell injection layer,transition layer and the main fluid channel layer,the main fluid channel layer is the main structure of the chip,including cell culture exchange of inward and outward and the structure of the shunt,reduce disturbance effect.Simulation analysis was carried out on the institutions,it is proved in this paper,the design of microfluidic chip has the stable velocity field,to be able to provide stable microenvironment for cell growth.(3)Verify the structure stability of the velocity field.A method of particle velocity measurement was used to analyze the kinematics of the microfluid in the culture chamber.After the image processing to obtain the average speed of the chip culture chamber,and comparison of different structure,validation of mesh structure can provide more stable velocity field.With the increase of degree of mesh,the environment of the culture chamber is more stable.Hela cells culture experiments on these chips to verify the application of this design.