Application Of Aberration Compensation Based On LC-SLM For A Spiral Phase Contrast Imaging

Spiral phase contrast imaging in microscopy is rising technology in recent years based on microscopic image with a spiral phase filter, to achieve edge contrast enhancement for both amplitude and phase object. On this basis, the spiral phase contrast imaging with appropriate improvements can realize quantitative imaging for phase object. The reflective liquid crystal spatial light modulator is an important device used in spiral phase contrast imaging, its reflective surface often uneven that can cause additional system aberration. At the same time, optical system with various lens itself also cause an wavefront aberrations, this could decline performance or accuracy of the quantitative imaged by spiral phase contrast microscopy.In this paper we presents a method based on Gerchberg-Saxton phase retrieval algorithm to caculate the wavefront aberration caused by the optical system and the uneven reflective surface of the reflective liquid crystal spatial light modulator, and then compensating this wavefront aberrations. This kind of wavefront aberration compensation method that based on Gerchberg-Saxton phase retrieval algorithm for the reflective liquid crystal spatial light modulator is applied to a quantitative imaging of spiral phase contrast microscopy, through this process the image clarity of the system for polystyrene microsphere sample has been improved. With the numerical treatment of the interference graph which imaged by the quantitative spiral phase contrast microscopy for polystyrene microsphere sample, the results confirm that the accuracy of this spiral phase contrast microscopy imaging system has been improved after compensating this wavefront aberration.