Effects Of Exercise Combined With Dietary Interventionon Physical Fitness And Oxidative Stress In Obese Female Adolescents

Objective: To investigate effects of 4 weeks closed-weight-loss training combined with diet intervention on obese female adolescents body shape and physical fitness and oxidative stress.This study aims to provide basis reference for prevention from obesity in female adolescent.Methods : 9 participants of female adolescents aged between 12-18 years old categorized as experimental group attended 4 weeks of closed-weight-loss training.Interventions were given 3 times/day,6 days/week until 4 weeks of low intensity aerobic exercise and healthy diet.There was observation before and after intervention in regard of body shape,physical fitness,and changes on oxidative stress index.Measurement were taken on body height,body weight,body fat mass,body fat percentage,protein content,body mass index,body composition index,sit and reach test,abdominal circumference,20 m of shuttle run,grip strength and physical indicators of oxidative stress markers as follow 8-iso-PGF2α、8-OHdG、T-AOC、CAT.Meanwhile,oxidative stress index measurement also conducted toward control group,10 wrestling female athletes with the same age as individuals on experimental group,without any of intervention involved.Results:(1)The average body weight of female obese adolescents decreased from 90.19 ± 15.11(kg)to 81.60 ± 12.85(kg),and the average body fat mass decreased from 34.64 ± 3.98(kg)to 28.32 ± 6.98(kg)(P<0.01);BMI decreased from 32.93 ± 4.84(kg / m2)to 28.84 ± 4.02(kg / m2)(P <0.01);Body fat percentage is extremely significant decline(P<0.01);And limb and torso fat content decreased evenly(P <0.01);The average total protein content of the body was 10.49 ± 1.20(kg)to 10.31 ± 1.11(kg)before and after intervention,but it was not statistically significant(P<0.01).(2)Results of sit and reach test before and after intervention for obese female adolescent is increase from 12.27±6.20 cm to 13.51±5.90cm(P>0.05);abdominal circumference and 20 m shuttle run significantly increase(P<0.01).(3)obese female adolescent before and after intervention T-AOC(12.36±5.1U/ml3 vs.17.57±3.13U/ml and CAT(0.80±0.16U/mlvs.1.83±0.58U/ml)showing remarkable improvement.8-iso-PGF2α(52.50±7.06 pg/ml vs.50.16±4.60 pg/ml)、8-OHdG(6.02±1.88 ng/ml vs.5.89±1.25 ng/ml)no significant change in level(P>0.05).(4)obese female adolescent and athletes comparison on oxidative stress index 8-OHdG、8-iso-PGF2α、T-AOC、CAT showing no significant difference(P>0.05);obese female adolescent and athletes comparison on antioxidant bio marker T-AOC(17.57±3.13 U/ml vs.13.01±1.81U/ml)and CAT(1.83±0.58U/mlvs.0.70±0.38U/ml)showing a significant difference(P<0.01);oxidative damage markers 8-iso-PGF2α Level is lower(P<0.05)、8-OHd G showing no significant difference(P>0.05).Conclusion:(1)4 weeks closed-weight-loss training combine with diet intervention can be used to improve body shape on obese female adolescent,significantly reduced the degree of obesity,and produce good weight loss effect.(2)4 weeks closed-weight-loss training combine with diet intervention can be used to improve the physical quality of obese female adolescent,especially cardiopulmonary action and muscle endurance.(3)4 weeks closed-weight-loss training combine with diet intervention can be used to significantly improve oxidative stress in obese female adolescent,especially to enhance the body’s antioxidant defense mechanism to achieve the improvement of stress state.