Effects Of Yangying Huoxue Decdoction On The Wnt/?-catenin Signaling Pathway In Rats With Hepatic Fibrosis

Objective1.Based on the Wnt/?-catenin signaling pathway,the effects of Yangying Huoxue decoction on Wnt1,?-catenin and TGF-?₁ were studied by detecting various indicators.And,to explore the mechanism of Yangying Huoxue decoction anti-liver fibrosis.2.To clarify the molecular mechanism of Yangying Huoxue decoction anti-hepatic fibrosis,enrich the connotation of hepatic fibrosis,provide theoretical support and experimental basis for the study of anti-hepatic fibrosis anti-fibrosis.Methods1.60 SPF adult Wistar rats?weight:180²20g?,female and male are each half,randomly divided into 6 groups:blank group,model group,Yangying Huoxue decoction low dosage,medium dosage and high dosage treatment groups,colchicine treatment group.In addition to the blank group,the other 5groups adopted the compound liver fibrosis modeling method to establish the model?40%of CCL₄ is mixed with paraffin,and combined with pig serum?.The rat liver fibrosis model was established after 8 weeks.2.Give each group rats corresponding drug intervention treatment for 8weeks.3.After anesthesia,take the blood from the abdominal aorta.To detect the biochemical indexes of liver fibrosis in rat serum?ALT?AST?HA?LN?PC???-C?.Make pathological section of the liver tissue of the rats.After HE staining and Masson staining,observe and compare their pathological findings.Test the m RNA level of Wnt1,?-catenin,TGF-?₁,by using the RT-PCR method.Using Western blot method to detect Wnt1,?-catenin,and TGF-?₁ protein expression.Results1.Compared with the normal group,the pathological changes of liver tissue in the model group were obvious,ALT and AST content increased in serum.Compared with the model group,different doses of Yangying Huoxue decoction and colchicine could improve the pathological changes of liver tissue in liver fibrosis.The content of ALT and AST in serum decreased significantly,and the difference was statistically significant.?P<0.01?2.Different doses of Yangying Huoxue decoction and colchicine can reduce rats serum liver fibrosis markers of HA,LN,PC?,?-C values.And,the serum hepatic fibrosis index of rats in high-dose group was significantly lower than that in colchicine group.?P<0.05?3.Rt-PCR and Western blot results showed that:The m RNA and protein expression levels of Wnt1,?-catenin and TGF-?₁ in the liver tissues of rats treated with Yangying Huoxue decoction were lower than those in the model group. Moreover,the expression level of high-dose group was significantly lower than that of colchicine group.?P<0.01?ConclusionYangying Huoxue decoction could reduce or inhibit the activation of Wnt/?-catenin signaling pathway.The expression levels of Wnt1,?-catenin and TGF-?₁ m RNA and protein were decreased,and the serum indexes of liver fibrosis in rats were reduced to achieve the effect of anti-fibrosis,and the effect of Yangying Huoxue decoction high-dose group was more significant.