Bioinformatics Analysis Of Differentially Expressed MRNA In Exosomes Derived From Oral Squamous Cell Carcinoma

Objectives Using transcriptome analysis to compare the mRNA expression between the exosomes derived from tongue oral cell cancer cells(OSCC)and human immortalized oral epithelial cell(HOEC),thus paving way for the further exploring of etiology and development mechanism of OSCC.Methods High throughput sequencing technology was used to explore mRNA expression profile in the exosomes of OSCC and HOEC.The differently expressed genes were selected and analyzed by Gene Ontoloty(GO)analysis and KEGG enrichment analysis.Results 44699 mRNA detected in the exosomes of OSCC,44761 mRNA detected in the exosomes of HOEC,471 differently expressed mRNAs were found(234 mRNAs were up-regulated and 237 mRNAs were down-regulated).Bioinformatics analysis showed that differently expressed mRNAs played an important role in the biological regulation and pathway of OSCC.Conclusion Abundant exosomes can be secreted by OSCC and HOEC.The mRNA expression profiles of exosome from OSCC are quite different compared with that from HOEC,which plays an important role in the occurrence,development and metastasis of tumor.Exploring mRNA expression profiles of the exosomes derived from two different cells can provide new ideas and methods for understanding the evolutional mechanism of oral squamous cell cancer.