| Incidence of cardiovascular diseases is increasing rapidly these days,among which ischemic heart disease is on the top of the list.Data from basic research showed that microcirculation dysfunction would also participate in the process of atherosclerotic diseases in addition to coronary artery stenosis.With deep advancement of relative studies,microcirculation dysfunction was shown to also contribute to the initiation,development,diagnosis,and prognosis of such diseases.Shexiang Baoxin pills were proved to decrease the MACE and ameliorate the symptom of patients with coronary artery diseases,but the underlying mechanism is still elusive.Based on these,a question was proposed that whether Shexiang Baoxin pills could improve the microcirculation dysfunction or not? And if yes,what is the exact mechanism?Objective: Combination of injection LPS and myocardial infarct in mice,a new microcirculation dysfunction model was established.Using it,the effects and involved mechanisms of Shexiang Baoxin pills on improving microcirculation dysfunction were investigated,including changes of microcirculation velocity,microcirculation permeability,WBC rolling and adhesion,and microcirculation diameters,which will provide potential strategies for future clinic.Methods: 60 male mice were used,of which 10 were allocated into the sham operation group,and others received LAD ligation to establish the AMI models.5 were dead during and after the operation,and 45 were alive left.Another 5 were excluded from the following experiment for LVEF more than 45% revealed by ultrasound examination one week after operation.Remaining 40 mice were randomized into model and different dosages of SXBXW groups(low dosage 6mg/ml,middle 12mg/ml,high24mg/ml).Differences of the microcirculation velocity of mice among different groups were analyzed using Image Pro Plus software.And while the changes of CD11 b and CD62 L expression on WBCs were evaluated with flow cytometry.Results:1.During the modeling period,10 mice were dead or not satisfied with AMI diagnosis requirement.Remaining 40 mice were given LPS injection to induce severe microcirculation dysfunction,which implied that the successful rate of such model was80%;2.Compared with that of sham operation group,microcirculation velocities of mice among model group significantly decreased,and the decreasing extent could be up to78%(p<0.05),the opening microcirculation numbers decreased up to 33%(p<0.05),WBC rolling numbers increased up to 4.8 times of sham group(p<0.05),and microcirculation permeability increased up to 1.7 times(p<0.05).While the diameter of microcirculation did not change significantly;3.Compared with model group,mice receiving SXBXW were shown to be with greatly improved microcirculation,among which the velocities increased up to 4.43,4.76 and 4.8 times(p<0.05),the opening microcirculation numbers increased up to 1.32,1.36 and 1.4 times(p<0.05),the rolling WBC numbers decreased by 26%,34% and36%(p<0.05),and microcirculation permeability decreased by 26.3%,28.9% and34.2%(p<0.05).And the diameters of microcirculation among mice receiving high dosage of SXBXW was 1.04 times of those of model group(p<0.05);4.Compared with low dosage group,mice receiving high dosage of SXBXW were proved to be with significantly higher microcirculation velocity(1.08 times),increased microcirculation numbers(1.05 times),decreased WBC rolling(13.8%)(all of which p<0.05);5.SXBXW could significantly decrease the expressions of CD11 b but increase those of CD62 L on WBCs.Conclusion:1.Microcirculation dysfunction could be established by combination of AMI and LPS injection;2.SXBXW could effectively improve the microcirculation dysfunction induced by AMI and LPS;3.The involving mechanism might be the decreased expression of cell adhesion molecules such as CD11 b on WBC,which would decrease the rolling and adhesion of WBCs to abnormal endothelial cell layer of microcirculation. |
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